Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidyl choline | addition increases catalytic activity of wild-type nzyme by 50% | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
DELTA1-114 | D114 truncation mutant shows 6.8% residual activity compared to wild-type, Km (arachidonate) increased compared to wild-type. Vmax: 9.3% compared to wild-type (100%). Addition of phosphatidyl choline or Ca2+ increases catalytic activity only by 11% compared to 50% in wild-type. Truncated mutant exhibits impaired membrane binding properties | Mus musculus |
DELTA1-125 | I125 truncation mutant shows 1.8% residual activity compared to wild-type, addition of phosphatidyl choline or Ca2+ increases catalytic activity only by 11% compared to 50% in wild-type. Truncated mutant exhibits impaired membrane binding properties | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
arachidonate | wild-type, pH and temperature not specified in the publication | Mus musculus | |
0.06 | - |
arachidonate | mutant DELTA1-114, pH and temperature not specified in the publication | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | addition of Ca2+ increases catalytic activity of wild-type nzyme by 50% | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | - |
Mus musculus | (5S,6E,8Z,11Z,14Z)-5-hydroperoxyicosa-6,8,11,14-tetraenoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5-LOX | - |
Mus musculus |