Literature summary for 1.13.11.34 extracted from

Michel, A.A.; Steinhilber, D.; Werz, O.
Development of a method for expression and purification of the regulatory C2-like domain of human 5-lipoxygenase (2008), Protein Expr. Purif., 59, 110-116.

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Activating Compound

Activating Compound	Comment	Organism	Structure
5-LO activating protein	i.e. FLAP, Ca2+ induces the translocation of 5-LO from a soluble compartment to nuclear structures, where 5-LO co-localizes with 5-LO activating protein	Homo sapiens
ATP	-	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression of regulatory C2-like domain of the enzyme, residues 1-128, containing a tobacco etch virus protease-cleavage site, in Escherichia coli strain BL21(DE3) as soluble maltose-binding-protein fusion protein	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
methanol	complete inhibition at 50% v/v	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	Ca2+ induces the translocation of 5-LO from a soluble compartment to nuclear structures, where 5-LO co-localizes with 5-LO activating protein, FLAP	Homo sapiens	5829	-
nucleus	Ca2+ induces the translocation of 5-LO from a soluble compartment to nuclear structures, where 5-LO co-localizes with 5-LO activating protein, FLAP	Homo sapiens	5634	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	activates, Ca2+ induces the translocation of 5-LO from a soluble compartment to nuclear structures, where 5-LO co-localizes with 5-LO activating protein, FLAP	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate	Homo sapiens	-	leukotriene A4 + H2O	is unstable and metabolized to leukotriene B4	?
arachidonic acid + O2	Homo sapiens	-	(6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	enzyme regulation by phosphorylation	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
recombinant soluble MBP-regulatory C2-like domain fusion protein from Escherichia coli strain BL21(DE3) by amylose affinity chromatography, fusion protein cleavage by TEV protease, and further purification of the C2-like domain by hydrophobic interaction chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate	-	Homo sapiens	leukotriene A4 + H2O	-	?
(6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate	-	Homo sapiens	leukotriene A4 + H2O	is unstable and metabolized to leukotriene B4	?
arachidonic acid + O2	-	Homo sapiens	(6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate	-	?

Subunits

Subunits	Comment	Organism
More	residues 1-128 of the enzyme form the regulatory C2-like domain	Homo sapiens

Synonyms

Synonyms	Comment	Organism
5-lipoxygenase	-	Homo sapiens
5-LO	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)