Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H362Q | reduced enzyme activity, similar amounts of iron as non-mutated enzyme | Homo sapiens |
H367N | inactive, contains 0.2 mol of iron per mol of protein, compared to 0.86 mol of iron per mol of protein for the wild-type enzyme | Homo sapiens |
H367Q | inactive, contains 0.5 mol of iron per mol of protein, compared to 0.86 mol of iron per mol of protein for the wild-type enzyme | Homo sapiens |
H367S | inactive, contains 0.5 mol of iron per mol of protein, compared to 0.86 mol of iron per mol of protein for the wild-type enzyme | Homo sapiens |
H372Q | no detectable enzyme activity, practically devoid of iron | Homo sapiens |
H390Q | reduced enzyme activity, similar amounts of iron as non-mutated enzyme | Homo sapiens |
H399Q | reduced enzyme activity, similar amounts of iron as non-mutated enzyme | Homo sapiens |
H550Q | no detectable enzyme activity, practically devoid of iron | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | wild-type enzyme contains 0.65 mol of iron per mol of enzyme, H372 and H550 constitute two of the iron ligands | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, stable for weeks | Homo sapiens |
4°C, stable for days | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | i.e. 5,8,11,14-eicosatetraenoic acid | Homo sapiens | (6E,8Z,11Z,14Z)-(5S)-hydroperoxyicosa-6,8,11,14-tetraenoate | - |
? |