Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.34 extracted from

  • Zhang, Y.Y.; Lind, B.; Raadmark, O.; Samuelsson, B.
    Iron content of human 5-lipoxygenase. Effects of mutations regarding conserved histidine residues (1993), J. Biol. Chem., 268, 2535-2541.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
H362Q reduced enzyme activity, similar amounts of iron as non-mutated enzyme Homo sapiens
H367N inactive, contains 0.2 mol of iron per mol of protein, compared to 0.86 mol of iron per mol of protein for the wild-type enzyme Homo sapiens
H367Q inactive, contains 0.5 mol of iron per mol of protein, compared to 0.86 mol of iron per mol of protein for the wild-type enzyme Homo sapiens
H367S inactive, contains 0.5 mol of iron per mol of protein, compared to 0.86 mol of iron per mol of protein for the wild-type enzyme Homo sapiens
H372Q no detectable enzyme activity, practically devoid of iron Homo sapiens
H390Q reduced enzyme activity, similar amounts of iron as non-mutated enzyme Homo sapiens
H399Q reduced enzyme activity, similar amounts of iron as non-mutated enzyme Homo sapiens
H550Q no detectable enzyme activity, practically devoid of iron Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Iron wild-type enzyme contains 0.65 mol of iron per mol of enzyme, H372 and H550 constitute two of the iron ligands Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, stable for weeks Homo sapiens
4°C, stable for days Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonate + O2 i.e. 5,8,11,14-eicosatetraenoic acid Homo sapiens (6E,8Z,11Z,14Z)-(5S)-hydroperoxyicosa-6,8,11,14-tetraenoate
-
?