Cloned (Comment) | Organism |
---|---|
expression in insect cells using a baculovirus expression vector, expression as an N-terminal fusion protein with a 31-amino acid polypeptide carrying a six-histidine tag and an enterokinase cleavage site | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,8,11,14-Eicosatetraynoic acid | IC50: 0.00006 mM | Sus scrofa | |
nordihydroguaiaretic acid | IC50: 0.0022 mM | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
arachidonic acid | - |
Sus scrofa | |
0.03 | - |
linoleic acid | - |
Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leukocyte | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.033 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
-70°C, 20% glycerol, 10 mM 2-mercaptoethanol, 0.3% Triton X-305, enzyme retains complete activity after 1 month | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic acid | Sus scrofa | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate | the 12-(S)-enantiomer is the predominant product | ? | |
linoleic acid + O2 | 19% of the activity with arachidonic acid | Sus scrofa | 13(S)-hydroperoxy-9Z,11E-octadecadienoic acid | - |
? |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00006 | - |
IC50: 0.00006 mM | Sus scrofa | 5,8,11,14-Eicosatetraynoic acid | |
0.0022 | - |
IC50: 0.0022 mM | Sus scrofa | nordihydroguaiaretic acid |