Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
36.3 | - |
3,4-dihydroxybenzoate | pH 7.4, 22°C | Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | Fe(III)-containing enzyme, 0.8 mol of iron per subunit | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63230 | - |
analytical ultracentrifugation | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoate + O2 | Aspergillus niger | - |
3-carboxy-cis,cis-muconate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.7 | - |
pH 7.4, 22°C | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoate + O2 | - |
Aspergillus niger | 3-carboxy-cis,cis-muconate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 35000, SDS-PAGE | Aspergillus niger |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.9 | - |
3,4-dihydroxybenzoate | pH 7.4, 22°C | Aspergillus niger |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetically distantly related to heterodimeric bacterial protocatechuate 3,4-dioxygenases | Aspergillus niger |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
3,4-dihydroxybenzoate | pH 7.4, 22°C | Aspergillus niger |