Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Geobacillus sp. | enzyme involved in last ring fission in naphtalene degradation, intermediates confirm proceeding through protocatechuic acid via ortho-claevage pathway | ? | - |
? | |
protocatechuic acid + O2 | Geobacillus sp. | - |
3-carboxy-cis,cis-muconic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus sp. | - |
thermophilic isolate G27 from high-temperature oilfield in Lithuania | - |
Purification (Comment) | Organism |
---|---|
cells harvested by centrifugation, washed twice with 50 mM sodium phosphate buffer, pH 7.0 containing 10% glycerol, disruption with solicitor, centrifuged, supernatant stored at -20°C | Geobacillus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
350 | - |
activity at the 10th h of the exponential growth phase | Geobacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme involved in last ring fission in naphtalene degradation, intermediates confirm proceeding through protocatechuic acid via ortho-claevage pathway | Geobacillus sp. | ? | - |
? | |
protocatechuic acid + O2 | - |
Geobacillus sp. | 3-carboxy-cis,cis-muconic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3,4-PCDase | - |
Geobacillus sp. |
protocatchetuate 3,4-dioxygenase | - |
Geobacillus sp. |