Application | Comment | Organism |
---|---|---|
environmental protection | the purified enzyme can be used in bioremediation of polluted groundwater or soil contaminated with various aromatic compounds ranging from monocyclic to polycyclic | Geobacillus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
3,4-dihydroxybenzoate | - |
Geobacillus sp. | |
0.033 | - |
catechol | - |
Geobacillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
480000 | - |
gel filtration | Geobacillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus sp. | - |
strain AY946034 | - |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture-condition:naphthalene-grown cell | - |
Geobacillus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
34.2 | - |
- |
Geobacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoate + O2 | - |
Geobacillus sp. | 3-carboxy-cis,cis-muconate | - |
? | |
catechol + O2 | - |
Geobacillus sp. | muconate | - |
? | |
additional information | no activity with gentisate | Geobacillus sp. | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Geobacillus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | 50°C: about 55% of maximal activity, 70°C: about 60% of maximal activity | Geobacillus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half-life is 40 min | Geobacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Geobacillus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0: about 40% of maximal activity, pH 9.0: about 65% of maximal activity | Geobacillus sp. |