Crystallization (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
- |
Acinetobacter sp. |
Protein Variants | Comment | Organism |
---|---|---|
DELTA319-322 | turnover-number is 4.14fold lower than that of the wild-type enzyme, the Km-value for 3,4-dihydroxybenzoate is 2.1fold lower than that of the wild-type enzyme | Acinetobacter sp. |
R133H | turnover-number is fold 500lower than that of the wild-type enzyme, the Km-value for 3,4-dihydroxybenzoate is 1.8fold higher than that of the wild-type enzyme | Acinetobacter sp. |
R457S | turnover-number is 1333fold lower than that of the wild-type enzyme, the Km-value for 3,4-dihydroxybenzoate is 2.2fold lower than that of the wild-type enzyme | Acinetobacter sp. |
Y408C | turnover-number is 523fold lower than that of the wild-type enzyme | Pseudomonas putida |
Y408E | turnover-number is 6800fold lower than that of the wild-type enzyme | Pseudomonas putida |
Y408H | turnover-number is 9714fold lower than that of the wild-type enzyme, the Km-value for 3,4-dihydroxybenzoate is 10fold lower than that of the wild-type enzyme | Pseudomonas putida |
Y447H | turnover-number is 567fold lower than that of the wild-type enzyme | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0029 | - |
3,4-dihydroxybenzoate | mutant enzyme Y447H | Pseudomonas putida | |
0.03 | - |
3,4-dihydroxybenzoate | wild-type enzyme | Pseudomonas putida | |
0.036 | - |
3,4-dihydroxybenzoate | mutant enzyme R457S | Acinetobacter sp. | |
0.038 | - |
3,4-dihydroxybenzoate | mutant enzyme DELTA319-322 | Acinetobacter sp. | |
0.056 | - |
O2 | wild-type enzyme | Pseudomonas putida | |
0.078 | - |
3,4-dihydroxybenzoate | wild-type enzyme | Acinetobacter sp. | |
0.142 | - |
3,4-dihydroxybenzoate | mutant enzyme R133H | Acinetobacter sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | presence of a alphabetaFe3+ protomer in a variety of stoichiometries | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. | - |
- |
- |
Pseudomonas putida | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoate + O2 | - |
Pseudomonas putida | 3-carboxy-cis,cis-muconate | - |
? | |
3,4-dihydroxybenzoate + O2 | - |
Acinetobacter sp. | 3-carboxy-cis,cis-muconate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
O2 | mutant enzyme Y408H | Pseudomonas putida | |
0.007 | - |
3,4-dihydroxybenzoate | mutant enzyme Y408H | Pseudomonas putida | |
0.01 | - |
O2 | mutant enzyme Y408E | Pseudomonas putida | |
0.01 | - |
3,4-dihydroxybenzoate | mutant enzyme Y408E | Pseudomonas putida | |
0.09 | - |
O2 | mutant enzyme R457S | Acinetobacter sp. | |
0.09 | - |
3,4-dihydroxybenzoate | mutant enzyme R457S | Acinetobacter sp. | |
0.12 | - |
O2 | mutant enzyme Y447H | Pseudomonas putida | |
0.12 | - |
3,4-dihydroxybenzoate | mutant enzyme Y447H | Pseudomonas putida | |
0.13 | - |
O2 | mutant enzyme Y408C | Pseudomonas putida | |
0.13 | - |
3,4-dihydroxybenzoate | mutant enzyme Y408C | Pseudomonas putida | |
0.24 | - |
O2 | mutant enzyme R133H | Acinetobacter sp. | |
0.24 | - |
3,4-dihydroxybenzoate | mutant enzyme R133H | Acinetobacter sp. | |
29 | - |
O2 | mutant enzyme DELTA319-322 | Acinetobacter sp. | |
29 | - |
3,4-dihydroxybenzoate | mutant enzyme DELTA319-322 | Acinetobacter sp. | |
68 | - |
O2 | wild-type enzyme | Pseudomonas putida | |
68 | - |
3,4-dihydroxybenzoate | wild-type enzyme | Pseudomonas putida | |
120 | - |
O2 | wild-type enzyme | Acinetobacter sp. | |
120 | - |
3,4-dihydroxybenzoate | wild-type enzyme | Acinetobacter sp. |