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Literature summary for 1.13.11.3 extracted from
- Immobilization of protocatechuate 3,4 -dioxygenase with activated agarose (1972), Biochim. Biophys. Acta, 289, 68-76.
General Stability
| General Stability | Organism |
|---|---|
| immobilization: slight increase of thermal stability, soluble and immobilized enzyme exhibit substantial activity in 2 M urea | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 61.6 | - |
- |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dihydroxybenzoate + O2 | - |
Pseudomonas aeruginosa | 3-carboxy-cis,cis-muconate | - |
? |  |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
immobilized enzyme shows slightly enhanced stability | Pseudomonas aeruginosa |
| 65 | - |
soluble and immobilized enzyme, 15 min, complete loss of activity | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 8.5 | immobilized enzyme | Pseudomonas aeruginosa |
| 8.3 | 8.4 | free enzyme | Pseudomonas aeruginosa |
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