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Literature summary for 1.13.11.28 extracted from
- 2,3-Dihydroxybenzoate 2,3-oxygease from the chloroplast fraction of Tecoma stans (1975), Phytochemistry, 14, 2135-2139.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| chloroplast enzyme more stable than soluble enzyme | Tecoma stans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 8-hydroxyquinoline | reversible by addition of Cu2+ | Tecoma stans |  |
| Ag+ | - |
Tecoma stans | |
| arsenite | - |
Tecoma stans | |
| azide | - |
Tecoma stans | |
| Cd2+ | - |
Tecoma stans | |
| CN- | - |
Tecoma stans | |
| Co2+ | - |
Tecoma stans | |
| diethyldithiocarbamate | reversible by addition of Cu2+ | Tecoma stans | |
| F- | - |
Tecoma stans | |
| Fe2+ | - |
Tecoma stans | |
| Fe3+ | - |
Tecoma stans | |
| glutathione | no inhibition of chloroplast enzyme | Tecoma stans | |
| Hg2+ | - |
Tecoma stans | |
| Mg2+ | no inhibition of chloroplast enzyme | Tecoma stans | |
| N-ethylmaleimide | - |
Tecoma stans | |
| Neocuproine | reversible by addition of Cu2+ | Tecoma stans | |
| p-chloromercuribenzoate | - |
Tecoma stans | |
| Salicylaldoxime | reversible by addition of Cu2+ | Tecoma stans | |
| Semicarbazide | - |
Tecoma stans | |
| Sulfide | - |
Tecoma stans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Tecoma stans | 9507 | - |
| soluble | - |
Tecoma stans | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-dihydroxybenzoate + O2 | Tecoma stans | enzyme of the beta-ketoadipate pathway | 2-carboxy-cis,cis-muconate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tecoma stans | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Tecoma stans | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-dihydroxybenzoate + O2 | - |
Tecoma stans | 2-carboxy-cis,cis-muconate | - |
? | |
| 2,3-dihydroxybenzoate + O2 | enzyme of the beta-ketoadipate pathway | Tecoma stans | 2-carboxy-cis,cis-muconate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Tecoma stans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
30% of activity | Tecoma stans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
- |
half-life of chloroplast enzyme is 8-9 h | Tecoma stans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
- |
Tecoma stans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
no activity above pH 7 | Tecoma stans |
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