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Literature summary for 1.13.11.28 extracted from
- A new mode of ring cleavage of 2,3-dihydroxybenzoic acid in Tecoma stans (L.). Partial purification and properties of 2,3-dihydroxybenzoate 2,3-oxygenase (1975), Eur. J. Biochem., 56, 163-171.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | preincubation with substrate protects against inactivation | Tecoma stans |  |
| 8-hydroxyquinoline | reversible by addition of Cu2+ | Tecoma stans | |
| Ag+ | - |
Tecoma stans | |
| azide | - |
Tecoma stans | |
| CN- | - |
Tecoma stans | |
| diethyldithiocarbamate | reversible by addition of Cu2+ | Tecoma stans | |
| dithiothreitol | preincubation with substrate protects against inactivation | Tecoma stans | |
| Fe2+ | - |
Tecoma stans | |
| Fe3+ | - |
Tecoma stans | |
| glutathione | reduced form, preincubation with substrate protects against inactivation | Tecoma stans | |
| Hg2+ | - |
Tecoma stans | |
| iodoacetate | preincubation with substrate protects against inactivation | Tecoma stans | |
| L-cysteine | preincubation with substrate protects against inactivation | Tecoma stans | |
| Mg2+ | - |
Tecoma stans | |
| N-ethylmaleimide | preincubation with substrate protects against inactivation | Tecoma stans | |
| Neocuproine | reversible by addition of Cu2+ | Tecoma stans | |
| p-chloromercuribenzoate | preincubation with substrate protects against inactivation | Tecoma stans | |
| Salicylaldoxime | reversible by addition of Cu2+ | Tecoma stans | |
| Sulfide | - |
Tecoma stans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 65 | - |
2,3-Dihydroxybenzoate | - |
Tecoma stans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Tecoma stans | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-dihydroxybenzoate + O2 | Tecoma stans | - |
2-carboxy-cis,cis-muconate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tecoma stans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Tecoma stans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Tecoma stans | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-dihydroxy-p-cumate + O2 | 28% of activity | Tecoma stans | 2-carboxy-4-isopropyl-cis,cis-muconate | - |
? | |
| 2,3-dihydroxy-p-toluate + O2 | 38% of activity | Tecoma stans | 2-carboxy-4-methyl-cis,cis-muconate | - |
? | |
| 2,3-dihydroxybenzoate + O2 | - |
Tecoma stans | 2-carboxy-cis,cis-muconate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Tecoma stans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| -20 | - |
cannot be stored at -20°C except as ammonium sulfate precipitate or adsorbed to tricalcium phosphate gel | Tecoma stans |
- |
- |
extremely labile, crude preparation loses 50% of activity within 3-4 h at pH 5.2 | Tecoma stans |
| 55 | - |
complete inactivation after 20 min, addition of 0.006 mM substrate protects against inactivation for 15 min | Tecoma stans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
- |
Tecoma stans |
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