Any feedback?
Literature summary for 1.13.11.27 extracted from
- Purification and some properties of human 4-hydroxyphenylpyruvate dioxygenase (I) (1977), J. Biol. Chem., 252, 5073-5084.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | reducing cofactor required, either ascorbate, or a combination of 2,6-dichlorophenol indophenol and glutathione in presence of catalase | Homo sapiens |  |
| glutathione | reducing cofactor required, either ascorbate, or a combination of 2,6-dichlorophenol indophenol and glutathione in presence of catalase | Homo sapiens | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 0.01 mM, 50% inhibition | Homo sapiens | |
| 1-(2'-thenonyl)-3,3,3-trifluoroacetone | 0.0049 mM, 50% inhibition | Homo sapiens | |
| 2,2'-bipyridyl | 1.8 mM, 50% inhibition | Homo sapiens | |
| 4-aminophenylpyruvate | competitive | Homo sapiens | |
| bathophenanthroline | 0.018 mM, 50% inhibition; reactivation to 30% by dialysis and to 60% by addition to Fe2+ | Homo sapiens | |
| Cupferron | 0.0011 mM, 50% inhibition | Homo sapiens | |
| diethyldithiocarbamate | 0.0049 mM, 50% inhibition; restored by dialysis | Homo sapiens | |
| Dithizone | 0.14 mM, 50% inhibition | Homo sapiens | |
| Fe2+ | 1 mM, 30% inhibition | Homo sapiens | |
| H2O2 | 10 nM, 60% inhibition. 0.001 mM, complete inhibition | Homo sapiens | |
| phenylpyruvate | competitive | Homo sapiens | |
| YCl3 | 1 mM, 50% inhibition | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
4-hydroxyphenylpyruvate | - |
Homo sapiens | |
| 0.05 | - |
O2 | - |
Homo sapiens | |
| 0.5 | - |
phenylpyruvate | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
2 * 43000, SDS-PAGE | Homo sapiens |
| 66000 | - |
gel filtration | Homo sapiens |
| 87000 | - |
equilibrium sedimentation | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.53 | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -60°C, pH 7.5, 3 months, 20% loss of activity when enzyme concentration is 0.5 g/L in Tris/HCl buffer, no loss of activity after 4 years when enzyme concentration is 5 g/l | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-hydroxyphenylpyruvate + O2 | activity with the keto-form of 4-hydroxyphenylpyruvate is 40times higher than with the enol tautomer | Homo sapiens | homogentisate + CO2 | - |
? | |
| phenylpyruvate + O2 | - |
Homo sapiens | 2-hydroxyphenylacetate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 43000, SDS-PAGE | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
and a second optimum at pH 7.8 | Homo sapiens |
| 7.8 | - |
and a second optimum at pH 4.5 | Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 10 | 37°C, 30 min, in presence of Fe2+, stable | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
