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Literature summary for 1.13.11.27 extracted from
- 4-Hydroxyphenylpyruvate dioxygenase from human liver (1987), Methods Enzymol., 142, 139-142.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2,6-dichlorophenol indophenol | reducing cofactor required, either ascorbate, or a combination of 2,6-dichlorophenol indophenol and glutathione | Homo sapiens |  |
| ascorbate | reducing cofactor required, either ascorbate, or a combination of 2,6-dichlorophenol indophenol and glutathione in presence of catalase | Homo sapiens | |
| glutathione | reducing cofactor required, either ascorbate, or a combination of 2,6-dichlorophenol indophenol and glutathione in presence of catalase | Homo sapiens | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+-chelators | - |
Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Homo sapiens | |
| 0.05 | - |
O2 | - |
Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | restores activity after inactivation by Fe2+-chelators | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
2 * 43000, SDS-PAGE | Homo sapiens |
| 87000 | - |
equilibrium sedimentation | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -60°C, stable for several months | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-hydroxyphenylpyruvate + O2 | - |
Homo sapiens | homogentisate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 43000, SDS-PAGE | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
and a second optimum at pH 7.8 | Homo sapiens |
| 7.8 | - |
and a second optimum at pH 4.5 | Homo sapiens |
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Release 2023.1 (January 2023)
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