Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. FLA | A2VA43 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
QueD | - |
Streptomyces sp. FLA |
quercetinase | - |
Streptomyces sp. FLA |
General Information | Comment | Organism |
---|---|---|
metabolism | the reaction takes place via three major steps: attack of the superoxide on the C2 of the substrate pyrone ring to generate a Ni(II)-peroxide intermediate, formation of the second C-O bond between C4 and the peroxide to produce a peroxide bridge, and simultaneous cleavage of the C2-C3, C3-C4, and O1-O2 bonds with the formation of 2-protocatechuoylphloroglucinol carboxylic acid and carbon monoxide. The third step is rate-limiting, with a barrier of 17.4 kcal/mol. For the second C-O bond formation, an alternative pathway is that the peroxide attacks the C3 of the substrate pyrone ring, leading to the formation of a four-membered ring intermediate, which then undergoes concerted C2-C3 and O1-O2 bond cleavages to produce an alpha-keto acid. This pathway is associated with a barrier of 30.6 kcal/mol. When Glu74 is protonated, the 2,3-dioxygenolytic pathway, however, has a lower barrier (21.8 kcal/mol) than the 2,4-dioxygenolytic pathway | Streptomyces sp. FLA |