Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli or in in Streptomyces lividans TK23 | Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0141 | - |
quercetin | - |
Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23041 | - |
x * 23041, calculated from sequence | Streptomyces sp. |
63000 | - |
gel filtration | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | A2VA43 | - |
- |
Streptomyces sp. FLA / DSM 41951 | A2VA43 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant QueD protein with a C-terminal hexahistidine-tag | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fisetin + O2 | i.e. 3,7,3',4'-tetrahydroxyflavone, 23% of the activity with quercetin | Streptomyces sp. | ? | - |
? | |
fisetin + O2 | i.e. 3,7,3',4'-tetrahydroxyflavone, 23% of the activity with quercetin | Streptomyces sp. FLA / DSM 41951 | ? | - |
? | |
galangin + O2 | i.e. 3,5,7-trihydroxyflavone, 28% of the activity with quercetin | Streptomyces sp. | ? | - |
? | |
galangin + O2 | i.e. 3,5,7-trihydroxyflavone, 28% of the activity with quercetin | Streptomyces sp. FLA / DSM 41951 | ? | - |
? | |
kaempferol + O2 | i.e. 3,5,7,4'-tetrahydroxyflavone. 70% of the activity with quercetin | Streptomyces sp. | ? | - |
? | |
additional information | no activity with luteolin | Streptomyces sp. | ? | - |
? | |
additional information | no activity with luteolin | Streptomyces sp. FLA / DSM 41951 | ? | - |
? | |
morin + O2 | i.e. 3,5,7,2',4'-pentahydroxyflavone, 1.7% of the activity with quercetin | Streptomyces sp. | ? | - |
? | |
myricetin + O2 | i.e. 3,5,7,3',4',5'-hexahydroxyflavone. 49% of the activity with quercetin | Streptomyces sp. | ? | - |
? | |
myricetin + O2 | i.e. 3,5,7,3',4',5'-hexahydroxyflavone. 49% of the activity with quercetin | Streptomyces sp. FLA / DSM 41951 | ? | - |
? | |
quercetin + O2 | - |
Streptomyces sp. | 2-protocatechoylphloroglucinolcarboxylate + CO | - |
? | |
quercetin + O2 | - |
Streptomyces sp. FLA / DSM 41951 | 2-protocatechoylphloroglucinolcarboxylate + CO | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23041, calculated from sequence | Streptomyces sp. |
Synonyms | Comment | Organism |
---|---|---|
QueD | - |
Streptomyces sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.45 | - |
quercetin | - |
Streptomyces sp. |