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Literature summary for 1.13.11.24 extracted from
- Evidence for a new metal in a known active site: purification and characterization of an iron-containing quercetin 2,3-dioxygenase from Bacillus subtilis (2004), Protein Expr. Purif., 35, 131-141.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Tiron | 0.8 mM, 25% activation | Bacillus subtilis |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
General Stability
| General Stability | Organism |
|---|---|
| storage in buffer, loss of 25% of activity during 48 h, high concentrations of ammonium chloride stabilize, ascorbic acid and cysteine do not | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 0.21 mM, 46% residual activity | Bacillus subtilis | |
| 2,2'-dipyridyl | 0.18 mM, 57% residual activity | Bacillus subtilis | |
| diethyldithiocarbamate | 89 mM, 62% residual activity | Bacillus subtilis | |
| EDTA | 94 mM, 94% residual activity | Bacillus subtilis | |
| kojic acid | 5 mM, 50% residual activity | Bacillus subtilis | |
| O-ethylxanthate | 6 mM, 51% residual activity | Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0008 | - |
quercetin | pH 7.5, 25°C | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37640 | - |
x * 37640, MALDI-TOF, SDS-PAGE | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
expression in Escherichia coli | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.5 | - |
25°C, pH 7.5 | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| quercetin + O2 | - |
Bacillus subtilis | 2-protocatechoylphloroglucinolcarboxylate + CO | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37640, MALDI-TOF, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| YxaG | - |
Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
precipitates below | Bacillus subtilis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus subtilis | calculated | - |
4.9 |
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