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Literature summary for 1.13.11.24 extracted from
- Flavonol 2,4-dioxygenase from Aspergillus niger DSM 821, a type 2 CuII-containing glycoprotein (1999), Eur. J. Biochem., 263, 871-878.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diethyldithiocarbamate | - |
Aspergillus niger |  |
| kojic acid | - |
Aspergillus niger | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0066 | - |
quercetin | - |
Aspergillus niger | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu | 1-1.6 mol per mol enzyme, nonblue type 2 Cu2+ protein | Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26510 | - |
alpha 1 * 69574 + beta 1 * 51733 + gamma 1 * 26510, MALDI-TOF | Aspergillus niger |
| 51733 | - |
alpha 1 * 69574 + beta 1 * 51733 + gamma 1 * 26510, MALDI-TOF | Aspergillus niger |
| 69574 | - |
alpha 1 * 69574 + beta 1 * 51733 + gamma 1 * 26510, MALDI-TOF | Aspergillus niger |
| 135000 | - |
gel filtration | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| quercetin + O2 | Aspergillus niger | - |
2-protocatechoylphloroglucinolcarboxylate + CO | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
DSM 821, grown on rutin | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | N-linked oligo-mannose type glycan chains, 46-54% carbohydrate | Aspergillus niger |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 55.7 | - |
- |
Aspergillus niger |
Storage Stability
| Storage Stability | Organism |
|---|---|
| stable at -20°C | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| quercetin + O2 | - |
Aspergillus niger | 2-protocatechoylphloroglucinolcarboxylate + CO | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | alpha 1 * 69574 + beta 1 * 51733 + gamma 1 * 26510, MALDI-TOF | Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Aspergillus niger |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 6 | Britton-Robinson buffer, composed of H3PO4, acetic acid, H3BO3 | Aspergillus niger |
| 5.5 | 6.7 | 50 mM MES buffer | Aspergillus niger |
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Release 2023.1 (January 2023)
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