Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
comparison with mammalian cysteine dioxygenase. The overall active site geometry is conserved but the different substrate specificity may be related to replacement of an arginine by a glutamine in the active site | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8 | - |
3-Mercaptopropanoate | pH 7.5, 37°C | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | active site iron is coordinated by 3 histidines and 3 water molecules | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9I0N5 | cf. EC 1.13.11.91 | - |
Pseudomonas aeruginosa DSM 22644 | Q9I0N5 | cf. EC 1.13.11.91 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
no modification | no posttranslational modification observed | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-mercaptopropanoate + O2 | reaction of EC 1.13.11.91 | Pseudomonas aeruginosa | 3-sulfinopropanoate + H+ | - |
? | |
3-mercaptopropanoate + O2 | reaction of EC 1.13.11.91 | Pseudomonas aeruginosa DSM 22644 | 3-sulfinopropanoate + H+ | - |
? | |
L-cysteine + O2 | - |
Pseudomonas aeruginosa | 3-sulfinoalanine | - |
? | |
L-cysteine + O2 | - |
Pseudomonas aeruginosa DSM 22644 | 3-sulfinoalanine | - |
? | |
additional information | recombinant 3MDO is able to oxidize both cysteine and 3-mercaptopropionic acid in vitro, with a marked preference for 3-mercaptopropionic acid. Substrate binding to the ferrous iron is through the thiol but each substrate may adopt different coordination geometries | Pseudomonas aeruginosa | ? | - |
- |
|
additional information | recombinant 3MDO is able to oxidize both cysteine and 3-mercaptopropionic acid in vitro, with a marked preference for 3-mercaptopropionic acid. Substrate binding to the ferrous iron is through the thiol but each substrate may adopt different coordination geometries | Pseudomonas aeruginosa DSM 22644 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 23746, calculated from sequence | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
3MDO | - |
Pseudomonas aeruginosa |
PA2602 | - |
Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
3-Mercaptopropanoate | pH 7.5, 37°C | Pseudomonas aeruginosa |
Organism | Comment | Expression |
---|---|---|
Pseudomonas aeruginosa | low expression under standard growth conditions | additional information |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
3-Mercaptopropanoate | pH 7.5, 37°C | Pseudomonas aeruginosa |