Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethylxanthate | 0.002 mM, 110% of initial activity, 0.004 mM, 89% of initial activity | Cupriavidus necator |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Cupriavidus necator |
gene H16_A1614, located on chromosome 1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme CdoB in Escherichia coli | Cupriavidus necator |
gene H16_B1863, located on chromosome 2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme CdoA in Escherichia coli. Escherichia coli cells expressing CdoA can convert 3-mercaptopropionate to 3-sulfinopropionate | Cupriavidus necator |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Cupriavidus necator | |
3-Mercaptopropionate | complete inhibition at 0.05 mM | Cupriavidus necator | |
cysteamine | - |
Cupriavidus necator | |
EDTA | 0.05 mM, 80% of initial activity, 0.1 mM, 61% of initial activity; 40% inhibition at 0.1 mM, 65% inhibition at 0.2 mM; complete inhibition at 0.05 mM | Cupriavidus necator | |
ethyl xanthate | 44% inhibition at 0.004 mM | Cupriavidus necator | |
ethylxanthate | 0.002 mM, 110% of initial activity, 0.004 mM, 89% of initial activity | Cupriavidus necator | |
additional information | metal ions are inhibitory above 0.2 mM | Cupriavidus necator | |
sodium ethylxanthate | 11% inhibition at 0.004 mM | Cupriavidus necator |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.85 | - |
L-cysteine | pH 6.5, 30°C, recombinant His-tagged enzyme | Cupriavidus necator | |
5.7 | - |
3-Mercaptopropionate | pH 6.5, 30°C, recombinant His-tagged enzyme | Cupriavidus necator | |
6.7 | - |
3-Mercaptopropanoate | pH 6.5, 30°C | Cupriavidus necator | |
6.7 | - |
L-cysteine | pH 6.5, 30°C, recombinant His-tagged enzyme | Cupriavidus necator |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | activates | Cupriavidus necator | |
Fe2+ | 0.38 mol Fe(II) per mol CdoB, activates | Cupriavidus necator | |
Fe2+ | 0.42 mol Fe(II) per mol CdoB, activates | Cupriavidus necator | |
Fe3+ | 0.048 mol Fe(III) per mol enzyme | Cupriavidus necator | |
Iron | iron content of 0.38 mol Fe(II) per mol of enzyme | Cupriavidus necator | |
additional information | CdoA contains no Fe3+ | Cupriavidus necator | |
additional information | metal ions are inhibitory above 0.2 mM | Cupriavidus necator | |
Ni2+ | activates | Cupriavidus necator | |
Zn2+ | activates | Cupriavidus necator |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46800 | - |
gel filtration | Cupriavidus necator |
63900 | - |
recombinant His-tagged enzyme CdoB, gel filtration | Cupriavidus necator |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + O2 | Cupriavidus necator | - |
3-sulfinoalanine | - |
? | |
L-cysteine + O2 | Cupriavidus necator DSM 428 | - |
3-sulfinoalanine | - |
? | |
L-cysteine + O2 | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | - |
3-sulfinoalanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | Q0K029 | - |
- |
Cupriavidus necator | Q0K029 | cf. EC 1.13.11.91 | - |
Cupriavidus necator | Q0KB75 | - |
- |
Cupriavidus necator DSM 428 | Q0K029 | cf. EC 1.13.11.91 | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | Q0K029 | - |
- |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | Q0KB75 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme CdoA from Escherichia coli by nickel affinity chromatography to homogeneity | Cupriavidus necator |
recombinant His-tagged enzyme CdoB from Escherichia coli by nickel affinity chromatography to homogeneity | Cupriavidus necator |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.05 | - |
pH 6.5, 30°C | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-mercaptopropanoate + O2 | reaction of EC 1.13.11.91 | Cupriavidus necator | 3-sulfinopropanoate + H+ | - |
? | |
3-mercaptopropanoate + O2 | reaction of EC 1.13.11.91 | Cupriavidus necator DSM 428 | 3-sulfinopropanoate + H+ | - |
? | |
3-mercaptopropanoate + O2 | reaction of EC 1.13.11.91 | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | 3-sulfinopropanoate + H+ | - |
? | |
3-mercaptopropionate + O2 | 3fold higher activity compared to L-cysteine. 3-Mercaptopropionate does not occur naturally in cells of strain H16 | Cupriavidus necator | 3-sulfinopropionate | - |
? | |
3-mercaptopropionate + O2 | 3fold higher activity compared to L-cysteine. 3-Mercaptopropionate does not occur naturally in cells of strain H16 | Cupriavidus necator DSM 428 | 3-sulfinopropionate | - |
? | |
3-mercaptopropionate + O2 | 3fold higher activity compared to L-cysteine. 3-Mercaptopropionate does not occur naturally in cells of strain H16 | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | 3-sulfinopropionate | - |
? | |
L-cysteine + O2 | - |
Cupriavidus necator | 3-sulfinoalanine | - |
? | |
L-cysteine + O2 | - |
Cupriavidus necator DSM 428 | 3-sulfinoalanine | - |
? | |
L-cysteine + O2 | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | 3-sulfinoalanine | - |
? | |
additional information | no activity with 3-mercaptopropionate by CdoB | Cupriavidus necator | ? | - |
? | |
additional information | 3-mercaptopropanoate is preferred over cysteine. No substrate: cysteamine | Cupriavidus necator | ? | - |
- |
|
additional information | 3-mercaptopropanoate is preferred over cysteine. No substrate: cysteamine | Cupriavidus necator DSM 428 | ? | - |
- |
|
additional information | 3-mercaptopropanoate is preferred over cysteine. No substrate: cysteamine | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | ? | - |
- |
|
additional information | no activity with 3-mercaptopropionate by CdoB | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 22580, sequence calculation | Cupriavidus necator |
dimer | 2 * 22580, calculated from sequence | Cupriavidus necator |
trimer | 3 * 22390, sequence calculation | Cupriavidus necator |
Synonyms | Comment | Organism |
---|---|---|
CdoA | - |
Cupriavidus necator |
CdoB | - |
Cupriavidus necator |
H16_A1614 | - |
Cupriavidus necator |
H16_B1863 | - |
Cupriavidus necator |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Cupriavidus necator |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
3-Mercaptopropanoate | pH 6.5, 30°C | Cupriavidus necator | |
0.01 | - |
L-cysteine | pH 6.5, 30°C, recombinant His-tagged enzyme | Cupriavidus necator | |
0.25 | - |
L-cysteine | pH 6.5, 30°C, recombinant His-tagged enzyme | Cupriavidus necator | |
0.82 | - |
3-Mercaptopropionate | pH 6.5, 30°C, recombinant His-tagged enzyme | Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Cupriavidus necator |
6.8 | - |
- |
Cupriavidus necator |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cupriavidus necator | sequence calculation | - |
5.8 |
Cupriavidus necator | calculated from sequence | - |
5.8 |
Cupriavidus necator | sequence calculation | - |
7 |
General Information | Comment | Organism |
---|---|---|
malfunction | deletion of cdoA might enable increased synthesis of polythioesters | Cupriavidus necator |
physiological function | importance of CdoA for the metabolism of the sulfur compounds 3,3'-thiodipropionic acid and 3,3'-dithiodipropionic acid by further converting their degradation product, 3-mercaptopropionate | Cupriavidus necator |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
3-Mercaptopropanoate | pH 6.5, 30°C | Cupriavidus necator | |
0.002 | - |
L-cysteine | pH 6.5, 30°C, recombinant His-tagged enzyme | Cupriavidus necator | |
0.087 | - |
L-cysteine | pH 6.5, 30°C, recombinant His-tagged enzyme | Cupriavidus necator | |
0.144 | - |
3-Mercaptopropionate | pH 6.5, 30°C, recombinant His-tagged enzyme | Cupriavidus necator |