Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | protein expression of recombinant wild-type enzyme in HepG2/C3A cells increases by 160% when extracellular cysteine levels are increased from 0 to 1 mM cysteine | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expression in HepG2/C3A cell | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
H86A | expression of either the wild-type or a catalytically inactivated mutant H86A in HepG2/C3A cells which do not express endogenous cysteine dioxygenase protein and culture in different concentrations of extracellular cysteine. Wild-type enzyme, but not mutant H86A, is capable of reducing intracellular cysteine levels in cells incubated in physiologically relevant concentrations of cysteine. Wild-type enzyme also decreases the glutathione pool and potentiates the toxicity of CdCl2 | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CdCl2 | cells transfected with wild-type enzyme show an enhanced sensitivity to CdCl2 that is limited to cells cultured in medium with cysteine levels of 0.1 and 0.3mM | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22500 | - |
x * 22500, calculated | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 22500, calculated | Rattus norvegicus |