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Literature summary for 1.13.11.20 extracted from

  • Chai, S.C.; Bruyere, J.R.; Maroney, M.J.
    Probes of the catalytic site of cysteine dioxygenase (2006), J. Biol. Chem., 281, 15774-15779.
    View publication on PubMed
Search for more literature for 1.13.11.20

Activating Compound

Activating Compound Comment Organism Structure
cysteamine at a reaction time of 45 min, CDO activity increases almost 20times in the presence of 5.8 mM cysteamine Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in BL21(D3)pLysS cells containing the pET-14b/CDO-ORF plasmid Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
alpha-ketoglutarate alpha-ketoglutarate inhibits cysteine dioxygenase with 50% inhibition at 6.8 mM Rattus norvegicus
aspartic acid aspartic acid decreases enzyme activity to 50% at a concentration of 1.5 mM. Replacing the sulfydryl by the uncharged hydroxyl group of serine does not affect enzyme activity. Rattus norvegicus
azide inhibits with a 50% activity reduction at 1.4 mM Rattus norvegicus
cyanide inhibits with a 50% activity reduction at 2.7 mM Rattus norvegicus
Mercaptopropionic acid mercaptopropionic acid at a concentration of 1.2 mM inhibits cysteine dioxygenase activity by 50% Rattus norvegicus
additional information iodoacetamide has no effect on the enzyme activity; S-methylcysteine does not inhibit cysteine dioxygenase Rattus norvegicus
S-carboxymethylcysteine S-carboxymethylcysteine exhibits 50% inhibition at a concentration of 2.3 mM Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized nickel affinity chromatography Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
L-cysteine + O2 = 3-sulfinoalanine Upon binding substrate, the structure of the iron site is pertubed but is still consistent with six O/N donor, indicating that cysteine may bind to the iron center, but is not bound via the sulfur atom. Rattus norvegicus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + O2
-
 Rattus norvegicus 3-sulfino-L-alanine
-
 ?
additional information cysteamine does not serve as substrate, D-cysteine does not serve as substrate Rattus norvegicus ?
-
 ?

Synonyms

Synonyms Comment Organism
CDO
-
 Rattus norvegicus