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Literature summary for 1.13.11.2 extracted from

  • Iwaki, M.; Nozaki, M.
    Immobilization of metapyrocatechase and its properties in comparison with the soluble enzyme (1982), J. Biochem., 91, 1549-1553.
    View publication on PubMed
Search for more literature for 1.13.11.2

General Stability

General Stability Organism
immobilization enhances stability against inactivation by heat, acid or alkaline pH and various denaturing agents Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Pseudomonas putida
0.063
-
 O2 immobilized enzyme Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Oxidation Stability

Oxidation Stability Organism
like native enzyme the immobilized enzyme is rapidly inactivated by oxidants such as O2 or H2O2 Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
46
-
 immobilized enzyme Pseudomonas putida
270
-
 free enzyme Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-chlorocatechol + O2
-
 Pseudomonas putida 2-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
-
 ?
3-hydroxycatechol + O2
-
 Pseudomonas putida 3-hydroxymuconic acid
-
 ?
3-methylcatechol + O2
-
 Pseudomonas putida 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
4-methylcatechol + O2 highly specific for Pseudomonas putida 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
catechol + O2
-
 Pseudomonas putida 2-hydroxymuconate semialdehyde
-
 ?
protocatechualdehyde + O2
-
 Pseudomonas putida ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Pseudomonas putida