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Literature summary for 1.13.11.17 extracted from

  • Kunapuli, S.P.; Vaidyanathan, C.S.
    Indole oxygenase from the leaves of Tecoma stans (1982), Plant Sci. Lett., 24, 183-188.
No PubMed abstract available

General Stability

General Stability Organism
dialysis results in complete loss of activity Tecoma stans

Inhibitors

Inhibitors Comment Organism Structure
Atebrin no effect Tecoma stans
azide
-
Tecoma stans
Cu2+ 1 mM, CuSO4, 25% inhibition Tecoma stans
cyanide
-
Tecoma stans
diethyldithiocarbamate
-
Tecoma stans
Dithionite
-
Tecoma stans
Fe2+ 0.5 mM, 35% inhibition Tecoma stans
Salicylaldoxime
-
Tecoma stans

Organism

Organism UniProt Comment Textmining
Tecoma stans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Tecoma stans

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Tecoma stans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0157
-
disappearance of 1 nmol indole in 1 min Tecoma stans

Storage Stability

Storage Stability Organism
-20°C, 18 h without appreciable loss of activity, complete loss of activity after 48 h Tecoma stans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-bromoindole + O2
-
Tecoma stans 5-bromo-2-formylaminobenzaldehyde
-
?
5-hydroxyindole + O2
-
Tecoma stans 2-formylamino-5-hydroxybenzaldehyde
-
?
5-methylindole + O2
-
Tecoma stans 2-formylamino-5-methylbenzaldehyde
-
?
indole + O2 conversion of indole to anthranilic acid Tecoma stans anthranilic acid
-
?
o-aminobenzaldehyde + O2
-
Tecoma stans anthranilic acid
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Tecoma stans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
-
Tecoma stans

Cofactor

Cofactor Comment Organism Structure
FAD not a flavoprotein Tecoma stans