Cloned (Comment) | Organism |
---|---|
gene hpaD, gene cluster hpaG1G2EDFHI encodes the homoprotocatechuate pathway is located at C2 in strain LB400 genome, quantitative expression analysis | Paraburkholderia xenovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxyphenylacetate + O2 | Paraburkholderia xenovorans | - |
2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? | |
additional information | Paraburkholderia xenovorans | LB400 cells grown with 3-hydroxyphenylacetate degrade homoprotocatechuate and show homoprotocatechuate 2,3-dioxygenase activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paraburkholderia xenovorans | - |
gene hpaD | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:3-hydroxyphenylacetate-grown cell | - |
Paraburkholderia xenovorans | - |
culture condition:4-hydroxyphenylacetate-grown cell | - |
Paraburkholderia xenovorans | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxyphenylacetate + O2 | - |
Paraburkholderia xenovorans | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? | |
additional information | LB400 cells grown with 3-hydroxyphenylacetate degrade homoprotocatechuate and show homoprotocatechuate 2,3-dioxygenase activity | Paraburkholderia xenovorans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
homoprotocatechuate 2,3-dioxygenase | - |
Paraburkholderia xenovorans |
HPC 2,3-dioxygenase | - |
Paraburkholderia xenovorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Paraburkholderia xenovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Paraburkholderia xenovorans |
Organism | Comment | Expression |
---|---|---|
Paraburkholderia xenovorans | induction of fumarylacetoacetate hydrolase HmgB in Burkholderia xenovorans LB400 during growth on hydroxyphenylacetates | additional information |
Paraburkholderia xenovorans | LB400 cells grown with 3-hydroxyphenylacetate and 4-hydroxyphenylacetate both show induction of the homoprotocatechuate pathway, they degrade homoprotocatechuate and show homoprotocatechuate 2,3-dioxygenase activity. The hmgA1 gene is upregulated by 3-hydroxyphenylacetate in strain LB400. The expression of the hpaD gene, encoding HPC 2,3-dioxygenase, is induced in 3-hydroxyphenylacetate and 4-hydroxyphenylacetate grown-cells | up |