Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Sodium ascorbate | 1 mM, revers purification inactivation of Fe-MndD, about 30-35% higher catalytic activity than the purified enzyme | Arthrobacter globiformis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Brevibacterium fuscum |
expression in Escherichia coli DH5alpha | Arthrobacter globiformis |
Crystallization (Comment) | Organism |
---|---|
X-ray | Arthrobacter globiformis |
X-ray | Brevibacterium fuscum |
General Stability | Organism |
---|---|
Fe-HPCD stable in air for at least several days | Brevibacterium fuscum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
air | initially high activity of Fe-MndD, becomes inactivtated within one h | Arthrobacter globiformis | |
ferricyanide | 1 mM, inactivates Fe-MndD | Arthrobacter globiformis | |
ferricyanide | 1 mM, inactivates Fe-HPCD | Brevibacterium fuscum | |
H2O2 | 1 mM, inactivates Fe-MndD | Arthrobacter globiformis | |
H2O2 | 1 mM, inactivates Fe-HPCD | Brevibacterium fuscum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
3,4-Dihydroxyphenylacetate | Mn-MndD | Arthrobacter globiformis | |
0.015 | - |
3,4-Dihydroxyphenylacetate | Fe-MndD | Arthrobacter globiformis | |
0.031 | - |
3,4-Dihydroxyphenylacetate | Fe-HPCD | Brevibacterium fuscum | |
0.035 | - |
3,4-Dihydroxyphenylacetate | Mn-HPCD | Brevibacterium fuscum | |
0.037 | - |
O2 | Fe-MndD | Arthrobacter globiformis | |
0.05 | - |
O2 | Mn-HPCD | Brevibacterium fuscum | |
0.06 | - |
O2 | Fe-HPCD | Brevibacterium fuscum | |
0.062 | - |
O2 | Mn-MndD | Arthrobacter globiformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | incorporated in Fe-HPCD | Brevibacterium fuscum | |
Fe2+ | nonphysiological metal, incorporated in Fe-MndD | Arthrobacter globiformis | |
Fe3+ | nonphysiological metal, incorporated in Fe-MndD | Arthrobacter globiformis | |
Mn2+ | incorporated in Mn-MndD | Arthrobacter globiformis | |
Mn2+ | nonphysiological metal, incorporated in Mn-HPCD | Brevibacterium fuscum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter globiformis | - |
- |
- |
Brevibacterium fuscum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Arthrobacter globiformis |
- |
Brevibacterium fuscum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxyphenylacetate + O2 | 1 mM, assay at pH 7.8, 23°C | Arthrobacter globiformis | ? | - |
? | |
3,4-dihydroxyphenylacetate + O2 | 1 mM, assay at pH 7.8, 23°C | Brevibacterium fuscum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Fe-HPCD | Fe(II) incorporated | Brevibacterium fuscum |
Fe-MndD | Fe incorporated | Arthrobacter globiformis |
homoprotocatechuate 2,3-dioxygenase | - |
Arthrobacter globiformis |
homoprotocatechuate 2,3-dioxygenase | - |
Brevibacterium fuscum |
Mn-HPCD | Mn(II) incorporated | Brevibacterium fuscum |
Mn-MndD | Mn(II) incorporated | Arthrobacter globiformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Arthrobacter globiformis |
23 | - |
assay at | Brevibacterium fuscum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Arthrobacter globiformis |
7.8 | - |
assay at | Brevibacterium fuscum |