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Literature summary for 1.13.11.15 extracted from

  • Emerson, J.P.; Kovaleva, E.G.; Farquhar, E.R.; Lipscomb, J.D.; Que, L.
    Swapping metals in Fe- and Mn-dependent dioxygenases: evidence for oxygen activation without a change in metal redox state (2008), Proc. Natl. Acad. Sci. USA, 105, 7347-7352.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Sodium ascorbate 1 mM, revers purification inactivation of Fe-MndD, about 30-35% higher catalytic activity than the purified enzyme Arthrobacter globiformis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Brevibacterium fuscum
expression in Escherichia coli DH5alpha Arthrobacter globiformis

Crystallization (Commentary)

Crystallization (Comment) Organism
X-ray Arthrobacter globiformis
X-ray Brevibacterium fuscum

General Stability

General Stability Organism
Fe-HPCD stable in air for at least several days Brevibacterium fuscum

Inhibitors

Inhibitors Comment Organism Structure
air initially high activity of Fe-MndD, becomes inactivtated within one h Arthrobacter globiformis
ferricyanide 1 mM, inactivates Fe-MndD Arthrobacter globiformis
ferricyanide 1 mM, inactivates Fe-HPCD Brevibacterium fuscum
H2O2 1 mM, inactivates Fe-MndD Arthrobacter globiformis
H2O2 1 mM, inactivates Fe-HPCD Brevibacterium fuscum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
3,4-Dihydroxyphenylacetate Mn-MndD Arthrobacter globiformis
0.015
-
3,4-Dihydroxyphenylacetate Fe-MndD Arthrobacter globiformis
0.031
-
3,4-Dihydroxyphenylacetate Fe-HPCD Brevibacterium fuscum
0.035
-
3,4-Dihydroxyphenylacetate Mn-HPCD Brevibacterium fuscum
0.037
-
O2 Fe-MndD Arthrobacter globiformis
0.05
-
O2 Mn-HPCD Brevibacterium fuscum
0.06
-
O2 Fe-HPCD Brevibacterium fuscum
0.062
-
O2 Mn-MndD Arthrobacter globiformis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ incorporated in Fe-HPCD Brevibacterium fuscum
Fe2+ nonphysiological metal, incorporated in Fe-MndD Arthrobacter globiformis
Fe3+ nonphysiological metal, incorporated in Fe-MndD Arthrobacter globiformis
Mn2+ incorporated in Mn-MndD Arthrobacter globiformis
Mn2+ nonphysiological metal, incorporated in Mn-HPCD Brevibacterium fuscum

Organism

Organism UniProt Comment Textmining
Arthrobacter globiformis
-
-
-
Brevibacterium fuscum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Arthrobacter globiformis
-
Brevibacterium fuscum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dihydroxyphenylacetate + O2 1 mM, assay at pH 7.8, 23°C Arthrobacter globiformis ?
-
?
3,4-dihydroxyphenylacetate + O2 1 mM, assay at pH 7.8, 23°C Brevibacterium fuscum ?
-
?

Synonyms

Synonyms Comment Organism
Fe-HPCD Fe(II) incorporated Brevibacterium fuscum
Fe-MndD Fe incorporated Arthrobacter globiformis
homoprotocatechuate 2,3-dioxygenase
-
Arthrobacter globiformis
homoprotocatechuate 2,3-dioxygenase
-
Brevibacterium fuscum
Mn-HPCD Mn(II) incorporated Brevibacterium fuscum
Mn-MndD Mn(II) incorporated Arthrobacter globiformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
assay at Arthrobacter globiformis
23
-
assay at Brevibacterium fuscum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Arthrobacter globiformis
7.8
-
assay at Brevibacterium fuscum