Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Brevibacillus brevis | |
3,4-dihydroxybenzoate | - |
Brevibacillus brevis | |
3,4-dihydroxycinnamate | - |
Brevibacillus brevis | |
3,4-Dihydroxyphenylpropionate | - |
Brevibacillus brevis | |
4-methylcatechol | - |
Brevibacillus brevis | |
4-nitrocatechol | - |
Brevibacillus brevis | |
8-hydroxyquinoline | - |
Brevibacillus brevis | |
catechol | - |
Brevibacillus brevis | |
m-hydroxyphenylacetate | - |
Brevibacillus brevis | |
p-hydroxyphenylacetate | - |
Brevibacillus brevis | |
PCMB | - |
Brevibacillus brevis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the enzyme contains 0.04-0.2 gatom of iron | Brevibacillus brevis | |
Manganese | approximately 2 gatom of manganese per mol of protein | Brevibacillus brevis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
4 * 36000, SDS-PAGE | Brevibacillus brevis |
120000 | - |
equilibrium sedimentation | Brevibacillus brevis |
140000 | - |
gel filtration | Brevibacillus brevis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxyphenylacetate + O2 | Brevibacillus brevis | enzyme catalyzes the estradiol cleavage of catechols | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacillus brevis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Brevibacillus brevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxymandelate + O2 | 14% of the activity with 3,4-dihydroxyphenylacetate | Brevibacillus brevis | ? | - |
? | |
3,4-dihydroxyphenylacetate + O2 | - |
Brevibacillus brevis | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? | |
3,4-dihydroxyphenylacetate + O2 | enzyme catalyzes the estradiol cleavage of catechols | Brevibacillus brevis | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? | |
3,4-dihydroxyphenylpropionate + O2 | 5% of the activity with 3,4-dihydroxyphenylacetate | Brevibacillus brevis | 2-hydroxy-5-carboxyethylmuconate semialdehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 36000, SDS-PAGE | Brevibacillus brevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Brevibacillus brevis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
most stable at | Brevibacillus brevis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
m-hydroxyphenylacetate | - |
Brevibacillus brevis | |
0.3 | - |
3,4-dihydroxycinnamate | - |
Brevibacillus brevis | |
0.5 | - |
4-nitrocatechol | - |
Brevibacillus brevis | |
1 | - |
3,4-Dihydroxyphenylpropionate | - |
Brevibacillus brevis | |
1.5 | - |
4-methylcatechol | - |
Brevibacillus brevis | |
1.8 | - |
p-hydroxyphenylacetate | - |
Brevibacillus brevis | |
2.3 | - |
3,4-dihydroxybenzoate | - |
Brevibacillus brevis | |
3.8 | - |
catechol | - |
Brevibacillus brevis |