Any feedback?
Literature summary for 1.13.11.12 extracted from
- Overproduction, purification, and characterization of extracellular lipoxygenase of Pseudomonas aeruginosa in Escherichia coli (2013), Appl. Microbiol. Biotechnol., 97, 5793-5800.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | lipoxygenase is widely used in food industry to improve aroma, rheological, or baking properties of foods | Pseudomonas aeruginosa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression and secretion of extracellular enzyme in Escherichia coli strain Rosetta(DE3) using the enzyme's endogenous signal peptide | Pseudomonas aeruginosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0489 | - |
linoleate | pH 7.5, 25°C, recombinant enzyme | Pseudomonas aeruginosa |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pseudomonas aeruginosa | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | nonheme, iron-containing dioxygenase | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
x * 70000, SDS-PAGE | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| linoleate + O2 | Pseudomonas aeruginosa | - |
(9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate | - |
? | |
| linoleate + O2 | Pseudomonas aeruginosa BBE | - |
(9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
| Pseudomonas aeruginosa BBE | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant extracellular enzyme 13.5fold from Escherichia coli strain Rosetta (DE3) cell supernatant b two different steps of anion exchange chromatography | Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28.3 | - |
purified recombinant enzyme, pH 7.5, 25°C | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| linoleate + O2 | - |
Pseudomonas aeruginosa | (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate | - |
? | |
| linoleate + O2 | - |
Pseudomonas aeruginosa | (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate | product identification by HPLC of recombinant extracellular enzyme, overview | ? | |
| linoleate + O2 | - |
Pseudomonas aeruginosa BBE | (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate | - |
? | |
| linoleate + O2 | - |
Pseudomonas aeruginosa BBE | (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate | product identification by HPLC of recombinant extracellular enzyme, overview | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 70000, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| liipoxygenase | - |
Pseudomonas aeruginosa |
| LOX | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
recombinant enzyme | Pseudomonas aeruginosa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | activity range, overview | Pseudomonas aeruginosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 65 | purified extracellular enzyme, 25°C, 7 min, stable at up to 35°C, loss of 10% activity at 40°C, and of 20% at 50°C, 10% activity remaining at 60°C, inactivation at 65°C | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
recombinant enzyme | Pseudomonas aeruginosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | activity range, overview | Pseudomonas aeruginosa |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
