Cloned (Comment) | Organism |
---|---|
vector pQE30-LOX expressed in Escherichia coli M15 cells | Olea europaea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
nordihydroguaiaretic acid | is a noncompetitive inhibitor | Olea europaea | |
propyl gallate | is a competitive inhibitor | Olea europaea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1749 | - |
linoleic acid | pH 6.0, 25°C | Olea europaea | |
0.3437 | - |
linolenic acid | pH 6.0, 25°C | Olea europaea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
98000 | - |
x * 98000, SDS-PAGE and immunoblot | Olea europaea |
98400 | - |
x * 98400, calculated from sequence | Olea europaea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Olea europaea | B6D1W5 | - |
- |
Purification (Comment) | Organism |
---|---|
by Ni-affinity chromatography and gel filtration, 26fold | Olea europaea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | is mainly expressed at late developmental stages, two Lox genes expressed in black olives. Increase of the LOX activity at the end of the green stage and the turning stage of the olives, and the maximum is reached at the black stage | Olea europaea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
23.4 | - |
26fold purified enzyme, with linoleic acid as substrate | Olea europaea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleic acid + O2 | - |
Olea europaea | (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoic acid | the recombinant enzyme shows a dual positional specificity, as it forms both 9- and 13-hydroperoxy octadecadienoic acid in a ratio 2:1 | ? | |
linolenic acid + O2 | - |
Olea europaea | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 98000, SDS-PAGE and immunoblot | Olea europaea |
? | x * 98400, calculated from sequence | Olea europaea |
Synonyms | Comment | Organism |
---|---|---|
9/13-LOX | - |
Olea europaea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
47.78 | - |
linolenic acid | pH 6.0, 25°C | Olea europaea | |
48.16 | - |
linoleic acid | pH 6.0, 25°C | Olea europaea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Olea europaea |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00188 | - |
propyl gallate | pH 6.0, 25°C | Olea europaea | |
0.00409 | - |
nordihydroguaiaretic acid | pH 6.0, 25°C | Olea europaea |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Olea europaea | calculated from sequence | - |
5.95 |
General Information | Comment | Organism |
---|---|---|
physiological function | 9/13-LOX is associated with the ripening and senescence processes | Olea europaea |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
140 | - |
linolenic acid | - |
Olea europaea | |
280 | - |
linoleic acid | - |
Olea europaea |