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Literature summary for 1.13.11.1 extracted from

  • Pandeeti, E.V.; Siddavattam, D.
    Purification and characterization of catechol 1,2-dioxygenase from Acinetobacter sp. DS002 and cloning, sequencing of partial catA gene (2011), Indian J. Microbiol., 51, 312-318.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene catA, DNA and amino acid sequence determination and analysis Acinetobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
2 * 66000, SDS-PAGE Acinetobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
catechol + O2 Acinetobacter sp.
-
cis,cis-muconate
-
?
catechol + O2 Acinetobacter sp. DS002
-
cis,cis-muconate
-
?

Organism

Organism UniProt Comment Textmining
Acinetobacter sp.
-
gene catA
-
Acinetobacter sp. DS002
-
gene catA
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme to homogeneity from cell supernatant by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, folloed by gel filtration Acinetobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzene-1,2,4-triol + O2 16% of the activity with catechol Acinetobacter sp. ?
-
?
benzene-1,2,4-triol + O2 16% of the activity with catechol Acinetobacter sp. DS002 ?
-
?
catechol + O2
-
Acinetobacter sp. cis,cis-muconate
-
?
catechol + O2 best substrate Acinetobacter sp. cis,cis-muconate
-
?
catechol + O2
-
Acinetobacter sp. DS002 cis,cis-muconate
-
?
catechol + O2 best substrate Acinetobacter sp. DS002 cis,cis-muconate
-
?
additional information poor or no activity with 3-methylcatechol, 4-methylcatechol, and 4-nitrocatechol Acinetobacter sp. ?
-
?
additional information poor or no activity with 3-methylcatechol, 4-methylcatechol, and 4-nitrocatechol Acinetobacter sp. DS002 ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 66000, SDS-PAGE Acinetobacter sp.

Synonyms

Synonyms Comment Organism
C12O
-
Acinetobacter sp.

General Information

General Information Comment Organism
physiological function catechol 1,2-dioxygenase is the key enzyme in ortho pathway of catechol degradation Acinetobacter sp.