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Literature summary for 1.13.11.1 extracted from
- A gene cluster involved in degradation of substituted salicylates via ortho cleavage in Pseudomonas sp. strain MT1 encodes enzymes specifically adapted for transformation of 4-methylcatechol and 3-methylmuconate (2007), J. Bacteriol., 189, 1664-1674.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene catA, DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic tree, identification of the cat gene cluster of strain MT1 | Pseudomonas reinekei |
| gene salD, DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic tree | Pseudomonas reinekei |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0008 | - |
catechol | pH not specified in the publication, 25°C | Pseudomonas reinekei |  |
| 0.001 | - |
3-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
| 0.001 | - |
4-Chlorocatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
| 0.0017 | - |
4-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
| 0.0056 | - |
3-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
| 0.0075 | - |
catechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
| 0.0099 | - |
4-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
x * 33000, SDS-PAGE, x * 34233, sequence calculation | Pseudomonas reinekei |
| 34209 | - |
x * 34209, sequence calculation | Pseudomonas reinekei |
| 34233 | - |
x * 33000, SDS-PAGE, x * 34233, sequence calculation | Pseudomonas reinekei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylcatechol + O2 | Pseudomonas reinekei | - |
(2Z,4Z)-3-methylhexa-2,4-dienedioate | - |
? | |
| 4-methylcatechol + O2 | Pseudomonas reinekei | - |
3-methyl-cis,cis-hexadienedioate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas reinekei | A0F0B5 | gene cat; encoded by the cat gene cluster, gene catA | - |
| Pseudomonas reinekei | Q0VH42 | gene salD; encoded by the cat gene cluster, gene catA | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:5-chlorosalicylate-grown cell | - |
Pseudomonas reinekei | - |
| culture condition:salicylate-grown cell | - |
Pseudomonas reinekei | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.2 | - |
pH not specified in the publication, 25°C, substrate 4-chlorocatechol | Pseudomonas reinekei |
| 1.4 | - |
pH not specified in the publication, 25°C, substrate 3-methylcatechol | Pseudomonas reinekei |
| 2.9 | - |
pH not specified in the publication, 25°C, substrate 4-chlorocatechol | Pseudomonas reinekei |
| 3.8 | - |
pH not specified in the publication, 25°C, substrate 3-methylcatechol | Pseudomonas reinekei |
| 4.4 | - |
pH not specified in the publication, 25°C, substrate 4-methylcatechol | Pseudomonas reinekei |
| 12.3 | - |
pH not specified in the publication, 25°C, substrate catechol | Pseudomonas reinekei |
| 15.3 | - |
pH not specified in the publication, 25°C, substrate catechol | Pseudomonas reinekei |
| 35.8 | - |
pH not specified in the publication, 25°C, substrate 4-methylcatechol | Pseudomonas reinekei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-methylcatechol + O2 | - |
Pseudomonas reinekei | (2Z,4Z)-2-methylhexa-2,4-dienedioate | - |
? | |
| 4-chlorocatechol + O2 | - |
Pseudomonas reinekei | (2E,4Z)-3-chlorohexa-2,4-dienedioate | - |
? | |
| 4-methylcatechol + O2 | - |
Pseudomonas reinekei | (2Z,4Z)-3-methylhexa-2,4-dienedioate | - |
? | |
| 4-methylcatechol + O2 | preferred substrate | Pseudomonas reinekei | (2Z,4Z)-3-methylhexa-2,4-dienedioate | - |
? | |
| 4-methylcatechol + O2 | - |
Pseudomonas reinekei | 3-methyl-cis,cis-hexadienedioate | - |
? | |
| catechol + O2 | - |
Pseudomonas reinekei | cis,cis-muconate | - |
? | |
| additional information | no activity with 3-chlorocatechol | Pseudomonas reinekei | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33000, SDS-PAGE, x * 34233, sequence calculation | Pseudomonas reinekei |
| ? | x * 34209, sequence calculation | Pseudomonas reinekei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| C12O | - |
Pseudomonas reinekei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7 | - |
4-Chlorocatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
| 0.8 | - |
3-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
| 1.7 | - |
4-Chlorocatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
| 2.3 | - |
3-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
| 2.7 | - |
4-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
| 7.5 | - |
catechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
| 8.7 | - |
catechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
| 20.7 | - |
4-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | 4- and 5-methylsalicylate as growth substrates are effectively converted by enzymes of the sal cluster into 4-methylmuconolactone, followed by isomerization to 3-methylmuconolactone. The function of the sal gene cluster is therefore to channel both chloro-substituted and methyl-substituted salicylates into a catechol ortho cleavage pathway, followed by dismantling of the formed substituted muconolactones through specific pathways | Pseudomonas reinekei |
| additional information | strain MT1 possesses two catechol 1,2-dioxygenases, encoded by the genes catA and salD, overview. The enzymes differ in their turnover rates for 4-chlorocatechol and especially for 4-methylcatechol, where C12OsalD exhibit significantly higher rates. Both enzymes also differ with respect to their apparent Km values | Pseudomonas reinekei |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0003 | - |
4-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
| 0.0004 | - |
3-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
| 0.0007 | - |
4-Chlorocatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
| 0.0008 | - |
3-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
| 0.001 | - |
catechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
| 0.0017 | - |
4-Chlorocatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
| 0.0109 | - |
catechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
| 0.0122 | - |
4-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
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