Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.1 extracted from

  • Pieper, D.H.; Stadler-Fritzsche, K.; Engesser, K.H.; Knackmuss, H.J.
    Metabolism of 2-chloro-4-methylphenoxyacetate by Alcaligenes eutrophus JMP 134 (1993), Arch. Microbiol., 160, 169-178.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,5-dichlorocatechol + O2 Cupriavidus necator reaction catalyzed by catechol 1,2-dioxygenase type II (2E,4E)-2,4-dichlorohexa-2,4-dienedioate
-
?
3,5-dichlorocatechol + O2 Cupriavidus necator JMP 134-1 reaction catalyzed by catechol 1,2-dioxygenase type II (2E,4E)-2,4-dichlorohexa-2,4-dienedioate
-
?
3-chloro-5-methylcatechol + O2 Cupriavidus necator reaction catalyzed by catechol 1,2-dioxygenase type II 2-chloro-4-methylhexa-2,4-dienedioate
-
?
3-chloro-5-methylcatechol + O2 Cupriavidus necator JMP 134-1 reaction catalyzed by catechol 1,2-dioxygenase type II 2-chloro-4-methylhexa-2,4-dienedioate
-
?
4-methylcatechol + O2 Cupriavidus necator reaction catalyzed by catechol 1,2-dioxygenase type I and type II 3-methylhexa-2,4-dienedioate
-
?
4-methylcatechol + O2 Cupriavidus necator JMP 134-1 reaction catalyzed by catechol 1,2-dioxygenase type I and type II 3-methylhexa-2,4-dienedioate
-
?
catechol + O2 Cupriavidus necator reaction catalyzed by catechol 1,2-dioxygenase type I cis,cis-muconate
-
?
catechol + O2 Cupriavidus necator JMP 134-1 reaction catalyzed by catechol 1,2-dioxygenase type I cis,cis-muconate
-
?

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
a spontaneous mutant of strain JMP 134, expressing the enzymes 2,4-D monooxygenase, chlorophenol hydroxylase, catechol 1,2-dioxygenase, dichloromuconate cycloisomerase, dienelactone hydrolase, and maleylacetate reductase constitutively
-
Cupriavidus necator JMP 134-1
-
a spontaneous mutant of strain JMP 134, expressing the enzymes 2,4-D monooxygenase, chlorophenol hydroxylase, catechol 1,2-dioxygenase, dichloromuconate cycloisomerase, dienelactone hydrolase, and maleylacetate reductase constitutively
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information 2-chloro-4-methylphenoxyacetate is no growth substrate for Alcaligenes eutrophus JMP 134 andJMP 134-1 Cupriavidus necator
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
whole cell induced by D-fructose, pH 7.4, 30°C Cupriavidus necator
0.19
-
whole cell induced by 2,4-dichlorophenoxyacetate, pH 7.4, 30°C Cupriavidus necator
0.41
-
whole cell induced by 2-chloro-4-methylphenoxyacetic acid, pH 7.4, 30°C Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,5-dichlorocatechol + O2 reaction catalyzed by catechol 1,2-dioxygenase type II Cupriavidus necator (2E,4E)-2,4-dichlorohexa-2,4-dienedioate
-
?
3,5-dichlorocatechol + O2 reaction catalyzed by catechol 1,2-dioxygenase type II Cupriavidus necator JMP 134-1 (2E,4E)-2,4-dichlorohexa-2,4-dienedioate
-
?
3-chloro-5-methylcatechol + O2 reaction catalyzed by catechol 1,2-dioxygenase type II Cupriavidus necator 2-chloro-4-methylhexa-2,4-dienedioate
-
?
3-chloro-5-methylcatechol + O2 reaction catalyzed by catechol 1,2-dioxygenase type II Cupriavidus necator JMP 134-1 2-chloro-4-methylhexa-2,4-dienedioate
-
?
4-methylcatechol + O2 reaction catalyzed by catechol 1,2-dioxygenase type I and type II Cupriavidus necator 3-methylhexa-2,4-dienedioate
-
?
4-methylcatechol + O2 reaction catalyzed by catechol 1,2-dioxygenase type I and type II Cupriavidus necator JMP 134-1 3-methylhexa-2,4-dienedioate
-
?
catechol + O2 reaction catalyzed by catechol 1,2-dioxygenase type I Cupriavidus necator cis,cis-muconate
-
?
catechol + O2 reaction catalyzed by catechol 1,2-dioxygenase type I Cupriavidus necator JMP 134-1 cis,cis-muconate
-
?

Synonyms

Synonyms Comment Organism
catechol 1,2-dioxygenase type I
-
Cupriavidus necator
catechol 1,2-dioxygenase type II
-
Cupriavidus necator

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
in vivo assay at Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
in vivo assay at Cupriavidus necator

Expression

Organism Comment Expression
Cupriavidus necator 2,4-dichlorophenoxyacetate and 2-chloro-4-methylphenoxyacetate induce the enzyme, 2-chloro-4-methylphenoxyacetate induces the enzymes of the chloroaromatic pathway, i.e. 2,4-D monooxygenase, chlorophenol hydroxylase, catechol 1,2-dioxygenase type II, dichloromuconate cycloisomerase, dienelactone hydrolase and maleylacetate reductase up

General Information

General Information Comment Organism
metabolism the enzyme is part of the chloroaromatic pathway and catalyzes the third step of the cometabolism of 2-chloro-4-methylphenoxyacetate by Alcaligenes eutrophus JMP 134 after growth on 2,4-dichlorophenoxyacetate, overview Cupriavidus necator