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Literature summary for 1.13.11.1 extracted from
- Purification and catalytic properties of two catechol 1,2-dioxygenase isozymes from benzoate-grown cells of Acinetobacter radioresistens (2000), J. Protein Chem., 19, 709-716.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Acinetobacter radioresistens |  |
| iodoacetamide | - |
Acinetobacter radioresistens | |
| o-phenanthroline | - |
Acinetobacter radioresistens | |
| Tiron | - |
Acinetobacter radioresistens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
catechol | IsoB | Acinetobacter radioresistens | |
| 0.0028 | - |
catechol | IsoA | Acinetobacter radioresistens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | isoenzyme A: 0.96 mol per mol enzyme, isoenzyme B: 0.89 mol per mol enzyme | Acinetobacter radioresistens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37700 | - |
2 * 37700, isoenzyme B, SDS-PAGE | Acinetobacter radioresistens |
| 38600 | - |
2 * 38600, isoenzyme A, SDS-PAGE | Acinetobacter radioresistens |
| 38600 | - |
3 * 38600, isoenzyme A, low ionic strength, SDS-PAGE | Acinetobacter radioresistens |
| 76200 | - |
isoenzyme B, gel filtration, high ionic strength | Acinetobacter radioresistens |
| 76800 | - |
isoenzyme B, gel filtration, low ionic strength | Acinetobacter radioresistens |
| 77600 | - |
isoenzyme A, gel filtration, high ionic strength | Acinetobacter radioresistens |
| 112400 | - |
isoenzyme A, gel filtration, low ionic strength | Acinetobacter radioresistens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter radioresistens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isoenzymes A and B | Acinetobacter radioresistens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Acinetobacter radioresistens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-methylcatechol + O2 | - |
Acinetobacter radioresistens | (2Z,4Z)-2-methylhexa-2,4-dienedioate | - |
? | |
| 4-methylcatechol + O2 | - |
Acinetobacter radioresistens | (2Z,4Z)-3-methylhexa-2,4-dienedioate | - |
? | |
| catechol + O2 | - |
Acinetobacter radioresistens | cis,cis-muconate | - |
? | |
| additional information | extradiol cleavage of 3-methylcatechol, no substrate: 3-chlorocatechol, protocatechuic acid | Acinetobacter radioresistens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 37700, isoenzyme B, SDS-PAGE | Acinetobacter radioresistens |
| homodimer | 2 * 38600, isoenzyme A, SDS-PAGE | Acinetobacter radioresistens |
| trimer | 3 * 38600, isoenzyme A, low ionic strength, SDS-PAGE | Acinetobacter radioresistens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 45 | IsoA | Acinetobacter radioresistens |
| 40 | - |
IsoB | Acinetobacter radioresistens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 31.3 | - |
catechol | isoenzyme B | Acinetobacter radioresistens | |
| 48.7 | - |
catechol | isoenzyme A | Acinetobacter radioresistens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | 8.5 | isoenzyme A | Acinetobacter radioresistens |
| 6.6 | 8.5 | isoenzyme B | Acinetobacter radioresistens |
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