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Literature summary for 1.13.11.1 extracted from

  • Murakami, S.; Wang, C.L.; Naito, A.; Shinke, R.; Aoki, K.
    Purification and characterization of four catechol 1,2-dioxygenase isoenzymes from the benzamide-assimilating bacterium Arthrobacter species BA-5-17 (1998), Microbiol. Res., 153, 163-171.
    View publication on PubMed
Search for more literature for 1.13.11.1

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
 Arthrobacter sp.
Cu2+
-
 Arthrobacter sp.
Fe2+ weak inactivation Arthrobacter sp.
Hg2+
-
 Arthrobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 1 mol per mol of subunit on the basis of the molecular mass of 33000 Da for each subunit Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 4 * 33000, SDS-PAGE Arthrobacter sp.
68000
-
 CD-I, CD-II, CD-III-2, gel filtration Arthrobacter sp.
72000
-
 CD-III-1, gel filtration Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
 BA-5-17
-
Arthrobacter sp. BA-5-17
-
 BA-5-17
-

Purification (Commentary)

Purification (Comment) Organism
four different isoenzymes: CD-I, DC-II, CD-III-1, CD-III-2 Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
36
-
 CD-III-2 Arthrobacter sp.
38
-
 CD-I Arthrobacter sp.
40
-
 CD-II Arthrobacter sp.
41
-
 CD-III-1 Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylcatechol + O2
-
 Arthrobacter sp. (2Z,4Z)-2-methylhexa-2,4-dienedioate
-
 ?
3-methylcatechol + O2
-
 Arthrobacter sp. BA-5-17 (2Z,4Z)-2-methylhexa-2,4-dienedioate
-
 ?
4-methylcatechol + O2
-
 Arthrobacter sp. (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
 ?
4-methylcatechol + O2
-
 Arthrobacter sp. BA-5-17 (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
 ?
catechol + O2
-
 Arthrobacter sp. cis,cis-muconate
-
 ?
catechol + O2
-
 Arthrobacter sp. BA-5-17 cis,cis-muconate
-
 ?
additional information intradiol cleavage of 3-and 4-methylcatechol, no extradiol cleavage of 3-methylcatechol or 3-chlorocatechol, slight intradiol cleavage activity for 3- and 4-substituted halocatechols, CD-II shows no activity for 4-chlorocatechol, no substrate: protocatechuic acid Arthrobacter sp. ?
-
 ?
additional information intradiol cleavage of 3-and 4-methylcatechol, no extradiol cleavage of 3-methylcatechol or 3-chlorocatechol, slight intradiol cleavage activity for 3- and 4-substituted halocatechols, CD-II shows no activity for 4-chlorocatechol, no substrate: protocatechuic acid Arthrobacter sp. BA-5-17 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 33000, SDS-PAGE Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
 CD-I, CD-II, CD-III-2 Arthrobacter sp.
8.9
-
 CD-III-1 Arthrobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.4 10 CD-III-1 Arthrobacter sp.
7.9 9 CD-I Arthrobacter sp.
8 10 CD-III-2 Arthrobacter sp.
8 8.6 CD-II Arthrobacter sp.