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Literature summary for 1.13.11.1 extracted from
- Purification and characterization of catechol 1,2-dioxygenase from Rhodococcus rhodochrous NCIMB 13259 and cloning and sequencing of its catA gene (1998), Biochem. J., 333, 741-747.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting-drop vapour-diffusion technique, X-ray characterization, space group C2, unit-cell dimensions: a = 111.9 a, b = 78.1 A, c = 134.6 A, beta = 100° | Rhodococcus rhodochrous |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
catechol | - |
Rhodococcus rhodochrous |  |
| 0.0012 | - |
4-methylcatechol | - |
Rhodococcus rhodochrous | |
| 0.0017 | - |
3-methylcatechol | - |
Rhodococcus rhodochrous | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | 0.60-0.65 iron per subunit | Rhodococcus rhodochrous | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31558 | - |
4 * 31558, electrospray mass spectrometry | Rhodococcus rhodochrous |
| 120000 | - |
gel filtration | Rhodococcus rhodochrous |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus rhodochrous | - |
NCIMB 13259 | - |
| Rhodococcus rhodochrous NCIMB 13259 | - |
NCIMB 13259 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhodococcus rhodochrous |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.3 | - |
- |
Rhodococcus rhodochrous |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-methylcatechol + O2 | - |
Rhodococcus rhodochrous | (2Z,4Z)-2-methylhexa-2,4-dienedioate | - |
? | |
| 3-methylcatechol + O2 | - |
Rhodococcus rhodochrous NCIMB 13259 | (2Z,4Z)-2-methylhexa-2,4-dienedioate | - |
? | |
| 4-methylcatechol + O2 | - |
Rhodococcus rhodochrous | (2Z,4Z)-3-methylhexa-2,4-dienedioate | - |
? | |
| 4-methylcatechol + O2 | - |
Rhodococcus rhodochrous NCIMB 13259 | (2Z,4Z)-3-methylhexa-2,4-dienedioate | - |
? | |
| catechol + O2 | - |
Rhodococcus rhodochrous | cis,cis-muconate | - |
? | |
| catechol + O2 | - |
Rhodococcus rhodochrous NCIMB 13259 | cis,cis-muconate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 31558, electrospray mass spectrometry | Rhodococcus rhodochrous |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.3 | - |
4-methylcatechol | - |
Rhodococcus rhodochrous | |
| 9.5 | - |
3-methylcatechol | - |
Rhodococcus rhodochrous | |
| 11.6 | - |
catechol | - |
Rhodococcus rhodochrous | |
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