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Literature summary for 1.13.11.1 extracted from
- Characterization of a chromosomally encoded catechol 1,2-dioxygenase (E.C. 1.13.11.1) from Alcaligenes eutrophus CH34 (1996), Arch. Microbiol., 166, 42-50.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3,4,5-trichlorocatechol | competitive inhibitor | Cupriavidus necator |  |
| 3-methylcatechol | at concentration at 20 microM and above | Cupriavidus necator | |
| 4,5-dichlorocatechol | competitive inhibitor | Cupriavidus necator | |
| tetrachlorocatechol | markedly inhibitory at concentration above 4 microM | Cupriavidus necator | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00025 | - |
catechol | - |
Cupriavidus necator | |
| 0.0005 | - |
4-Chlorocatechol | - |
Cupriavidus necator | |
| 0.0005 | - |
tetrachlorocatechol | - |
Cupriavidus necator | |
| 0.0007 | - |
4-Fluorocatechol | - |
Cupriavidus necator | |
| 0.002 | - |
3-chlorocatechol | - |
Cupriavidus necator | |
| 0.009 | - |
3-methylcatechol | - |
Cupriavidus necator | |
| 0.018 | - |
4-methylcatechol | - |
Cupriavidus necator | |
| 0.1 | - |
oxygen | - |
Cupriavidus necator | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | 0.83 mol per mol of protein | Cupriavidus necator | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
2 * 36000, SDS-PAGE | Cupriavidus necator |
| 76000 | - |
gel filtration | Cupriavidus necator |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cupriavidus necator | - |
Ralstonia eutropha CH34 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cupriavidus necator |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.36 | - |
- |
Cupriavidus necator |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-chlorocatechol + O2 | - |
Cupriavidus necator | 2-chloro-cis,cis-muconate | - |
? | |
| 3-methylcatechol + O2 | - |
Cupriavidus necator | (2Z,4Z)-2-methylhexa-2,4-dienedioate | - |
? | |
| 4-chlorocatechol + O2 | - |
Cupriavidus necator | (2E,4Z)-3-chlorohexa-2,4-dienedioate | - |
? | |
| 4-fluorocatechol + O2 | - |
Cupriavidus necator | 3-fluoro-cis,cis-muconate | - |
? | |
| 4-methylcatechol + O2 | - |
Cupriavidus necator | (2Z,4Z)-3-methylhexa-2,4-dienedioate | - |
? | |
| catechol + O2 | - |
Cupriavidus necator | cis,cis-muconate | - |
? | |
| tetrachlorocatechol + O2 | - |
Cupriavidus necator | cis,cis-tetrachloromuconic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 36000, SDS-PAGE | Cupriavidus necator |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
4-Chlorocatechol | - |
Cupriavidus necator | |
| 4 | - |
4-methylcatechol | - |
Cupriavidus necator | |
| 4 | - |
4-Fluorocatechol | - |
Cupriavidus necator | |
| 20 | - |
catechol | - |
Cupriavidus necator | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Cupriavidus necator |
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