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Literature summary for 1.13.11.1 extracted from

  • Aoki, K.; Konohana, T.; Shinke, R.; Nishira, H.
    Purification and characterization of catechol 1,2-dioxygenase from aniline-assimilating Rhodococcus erythropolis AN-13 (1984), Agric. Biol. Chem., 48, 2087-2095.
No PubMed abstract available
Search for more literature for 1.13.11.1

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
 Rhodococcus erythropolis
Fe2+
-
 Rhodococcus erythropolis
Fe3+
-
 Rhodococcus erythropolis
Hg2+
-
 Rhodococcus erythropolis
iodoacetic acid
-
 Rhodococcus erythropolis
o-phenanthroline
-
 Rhodococcus erythropolis
p-chloromercuribenzoate
-
 Rhodococcus erythropolis

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 1.3 gatom per mol enzyme protein Rhodococcus erythropolis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 4 * 36000, SDS-PAGE Rhodococcus erythropolis
37000
-
 both CD I and CD II, buffer containing NaCl, gel filtration Rhodococcus erythropolis
150000
-
 buffer omitting NaCl, gel filtration Rhodococcus erythropolis

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis
-
 AN-13
-
Rhodococcus erythropolis AN-13
-
 AN-13
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodococcus erythropolis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24
-
 catechol Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylcatechol + O2
-
 Rhodococcus erythropolis (2Z,4Z)-2-methylhexa-2,4-dienedioate
-
 ?
3-methylcatechol + O2
-
 Rhodococcus erythropolis AN-13 (2Z,4Z)-2-methylhexa-2,4-dienedioate
-
 ?
4-methylcatechol + O2
-
 Rhodococcus erythropolis (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
 ?
4-methylcatechol + O2
-
 Rhodococcus erythropolis AN-13 (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
 ?
catechol + O2
-
 Rhodococcus erythropolis cis,cis-muconate
-
 ?
catechol + O2
-
 Rhodococcus erythropolis AN-13 cis,cis-muconate
-
 ?
additional information intradiol cleavage of 3- and 4-methylcatechol, but not extradiol cleavage of 3-methylcatechol Rhodococcus erythropolis ?
-
 ?
additional information intradiol cleavage of 3- and 4-methylcatechol, but not extradiol cleavage of 3-methylcatechol Rhodococcus erythropolis AN-13 ?
-
 ?
pyrogallol + O2
-
 Rhodococcus erythropolis alpha-hydroxymuconic acid
-
 ?
pyrogallol + O2
-
 Rhodococcus erythropolis AN-13 alpha-hydroxymuconic acid
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 36000, SDS-PAGE Rhodococcus erythropolis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 for 10 min Rhodococcus erythropolis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 for catechol as substrate Rhodococcus erythropolis

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 10.5
-
 Rhodococcus erythropolis