Inhibitors | Comment | Organism | Structure |
---|---|---|---|
O2 | maintains 100% of its initial activity after a 48 h exposure to air, after 120 and 168 h exposures, the residual activities are 90% and 75%, respectively. It is very tolerant to oxygen | Aeropyrum camini | |
O2 | exhibits 80% residual activity after exposure to air for 24 h, decreases to 75% and 37% after 48 and 72 h exposures, respectively. After a 96 h exposure, activity is undetectable. The enzyme is oxygen sensitive | Persephonella hydrogeniphila |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Aeropyrum camini | - |
- |
soluble | - |
Persephonella hydrogeniphila | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
x * 85000, SDS-PAGE | Aeropyrum camini |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum camini | - |
- |
- |
Aeropyrum camini SY1 / ATCC BAA-758 / DSM 16960 / JCM 2091 | - |
- |
- |
Persephonella hydrogeniphila | - |
- |
- |
Persephonella hydrogeniphila 29W / DSM 15103 / JCM11663 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration, 55fold | Persephonella hydrogeniphila |
to homogeneity by gel filtration, 2.4fold | Aeropyrum camini |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.6 | - |
purified enzyme, proton reduction activity | Aeropyrum camini |
14.8 | - |
2.4fold purified enzyme, hydrogen oxidation activity | Aeropyrum camini |
50 | - |
- |
Persephonella hydrogeniphila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + oxidized methyl viologen | - |
Aeropyrum camini | H+ + reduced methyl viologen | - |
? | |
H2 + oxidized methyl viologen | - |
Aeropyrum camini SY1 / ATCC BAA-758 / DSM 16960 / JCM 2091 | H+ + reduced methyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 85000, SDS-PAGE | Aeropyrum camini |
Synonyms | Comment | Organism |
---|---|---|
hydrogenase II | - |
Aeropyrum camini |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
optimal temperature for the hydrogen oxidation activity and for the proton reduction activity | Aeropyrum camini |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
half-life of 48 h, shows hyperthermophily | Aeropyrum camini |
90 | - |
shows complete denaturation at 90°C after incubation for 30 min | Persephonella hydrogeniphila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
optimal pH for the proton reduction activity | Aeropyrum camini |
8.5 | - |
optimal pH for the hydrogen oxidation activity | Aeropyrum camini |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | optimal pH range for the hydrogen oxidation activity is 8.5 to 9.0, optimal pH range for the proton reduction activity is 6.0 to 6.5 | Aeropyrum camini |
General Information | Comment | Organism |
---|---|---|
physiological function | functions as a sensory hydrogenase | Aeropyrum camini |