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Literature summary for 1.12.99.6 extracted from

  • Nishimura, H.; Sako, Y.
    Purification and characterization of the oxygen-thermostable hydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum camini (2009), J. Biosci. Bioeng., 108, 299-303.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
O2 maintains 100% of its initial activity after a 48 h exposure to air, after 120 and 168 h exposures, the residual activities are 90% and 75%, respectively. It is very tolerant to oxygen Aeropyrum camini
O2 exhibits 80% residual activity after exposure to air for 24 h, decreases to 75% and 37% after 48 and 72 h exposures, respectively. After a 96 h exposure, activity is undetectable. The enzyme is oxygen sensitive Persephonella hydrogeniphila

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Aeropyrum camini
-
-
soluble
-
Persephonella hydrogeniphila
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
x * 85000, SDS-PAGE Aeropyrum camini

Organism

Organism UniProt Comment Textmining
Aeropyrum camini
-
-
-
Aeropyrum camini SY1 / ATCC BAA-758 / DSM 16960 / JCM 2091
-
-
-
Persephonella hydrogeniphila
-
-
-
Persephonella hydrogeniphila 29W / DSM 15103 / JCM11663
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration, 55fold Persephonella hydrogeniphila
to homogeneity by gel filtration, 2.4fold Aeropyrum camini

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.6
-
purified enzyme, proton reduction activity Aeropyrum camini
14.8
-
2.4fold purified enzyme, hydrogen oxidation activity Aeropyrum camini
50
-
-
Persephonella hydrogeniphila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + oxidized methyl viologen
-
Aeropyrum camini H+ + reduced methyl viologen
-
?
H2 + oxidized methyl viologen
-
Aeropyrum camini SY1 / ATCC BAA-758 / DSM 16960 / JCM 2091 H+ + reduced methyl viologen
-
?

Subunits

Subunits Comment Organism
? x * 85000, SDS-PAGE Aeropyrum camini

Synonyms

Synonyms Comment Organism
hydrogenase II
-
Aeropyrum camini

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
optimal temperature for the hydrogen oxidation activity and for the proton reduction activity Aeropyrum camini

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
half-life of 48 h, shows hyperthermophily Aeropyrum camini
90
-
shows complete denaturation at 90°C after incubation for 30 min Persephonella hydrogeniphila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
optimal pH for the proton reduction activity Aeropyrum camini
8.5
-
optimal pH for the hydrogen oxidation activity Aeropyrum camini

pH Range

pH Minimum pH Maximum Comment Organism
6 9 optimal pH range for the hydrogen oxidation activity is 8.5 to 9.0, optimal pH range for the proton reduction activity is 6.0 to 6.5 Aeropyrum camini

General Information

General Information Comment Organism
physiological function functions as a sensory hydrogenase Aeropyrum camini