Cloned (Comment) | Organism |
---|---|
overexpressed in a non-tagged version using plasmid pLO6, harboring the complete MBH operon, in Ralstonia eutropha strain HF631 | Cupriavidus necator H16 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | is oxygen-tolerant | Cupriavidus necator H16 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | the hydrogenase dimer is electronically connected to a membrane-integral cytochrome b | Cupriavidus necator H16 | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CN- | the catalytic center is equipped with 1.8 CN- per protein molecule | Cupriavidus necator H16 | |
CO | the catalytic center is equipped with one CO | Cupriavidus necator H16 | |
Fe | is composed of a small subunit, capable in coordinating one [3Fe4S] and two [4Fe4S] clusters | Cupriavidus necator H16 | |
Ni2+ | is composed of a large subunit, harboring the [NiFe] active site | Cupriavidus necator H16 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator H16 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by affinity chromatography, ca. 99% pure | Cupriavidus necator H16 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + oxidized acceptor | almost 100% of the membrane-attached MBH is reversibly redox-active | Cupriavidus necator H16 | H+ + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | detachment of the enzyme from the membrane | Cupriavidus necator H16 |
trimer | in its native environment | Cupriavidus necator H16 |
Synonyms | Comment | Organism |
---|---|---|
Mbh | - |
Cupriavidus necator H16 |
[NiFe] hydrogenase | - |
Cupriavidus necator H16 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
solubilization of the imer is associated with a downshift of the optimum pH for H2-oxidizing activity | Cupriavidus necator H16 |