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Literature summary for 1.12.99.6 extracted from

  • Mulder, D.W.; Ortillo, D.O.; Gardenghi, D.J.; Naumov, A.V.; Ruebush, S.S.; Szilagyi, R.K.; Huynh, B.; Broderick, J.B.; Peters, J.W.
    Activation of HydA(DeltaEFG) requires a preformed [4Fe-4S] cluster (2009), Biochemistry, 48, 6240-6248.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
HydE from Clostridium acetobutylicum extracts, activation of HydADELTAEFG requires the presence of a preformed [4Fe-4S] cluster on HydA Chlamydomonas reinhardtii
HydF from Clostridium acetobutylicum extracts, activation of HydADELTAEFG requires the presence of a preformed [4Fe-4S] cluster on HydA Chlamydomonas reinhardtii
HydG from Clostridium acetobutylicum extracts, activation of HydADELTAEFG requires the presence of a preformed [4Fe-4S] cluster on HydA Chlamydomonas reinhardtii

Cloned(Commentary)

Cloned (Comment) Organism
HydADELTAEFG cloned into a pET Duet vector and modified for the presence of an N-terminal six-histidine tag. Expressed in Escherichia coli BL21(DE3) cells Chlamydomonas reinhardtii

Metals/Ions

Metals/Ions Comment Organism Structure
Fe HydADELTAEFG binds a [4Fe-4S] cluster Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
x * 49000, SDS-PAGE, HydADELTAEFG Chlamydomonas reinhardtii

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HydADELTAEFG purified by anion exchange chromatography Chlamydomonas reinhardtii

Subunits

Subunits Comment Organism
? x * 49000, SDS-PAGE, HydADELTAEFG Chlamydomonas reinhardtii

Synonyms

Synonyms Comment Organism
hydA
-
Chlamydomonas reinhardtii