Activating Compound | Comment | Organism | Structure |
---|---|---|---|
HydE | from Clostridium acetobutylicum extracts, activation of HydADELTAEFG requires the presence of a preformed [4Fe-4S] cluster on HydA | Chlamydomonas reinhardtii | |
HydF | from Clostridium acetobutylicum extracts, activation of HydADELTAEFG requires the presence of a preformed [4Fe-4S] cluster on HydA | Chlamydomonas reinhardtii | |
HydG | from Clostridium acetobutylicum extracts, activation of HydADELTAEFG requires the presence of a preformed [4Fe-4S] cluster on HydA | Chlamydomonas reinhardtii |
Cloned (Comment) | Organism |
---|---|
HydADELTAEFG cloned into a pET Duet vector and modified for the presence of an N-terminal six-histidine tag. Expressed in Escherichia coli BL21(DE3) cells | Chlamydomonas reinhardtii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | HydADELTAEFG binds a [4Fe-4S] cluster | Chlamydomonas reinhardtii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
x * 49000, SDS-PAGE, HydADELTAEFG | Chlamydomonas reinhardtii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HydADELTAEFG purified by anion exchange chromatography | Chlamydomonas reinhardtii |
Subunits | Comment | Organism |
---|---|---|
? | x * 49000, SDS-PAGE, HydADELTAEFG | Chlamydomonas reinhardtii |
Synonyms | Comment | Organism |
---|---|---|
hydA | - |
Chlamydomonas reinhardtii |