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Literature summary for 1.12.99.6 extracted from

  • Laska, S.; Lottspeich, F.; Kletzin, A.
    Membrane-bound hydrogenase and sulfur reductase of the hyperthermophilic and acidophilic archaeon Acidianus ambivalens (2003), Microbiology, 149, 2357-2371.
    View publication on PubMed
Search for more literature for 1.12.99.6

Cloned(Commentary)

Cloned (Comment) Organism
-
 Acidianus ambivalens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane bound Acidianus ambivalens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe 0.134 mmol Fe per g protein Acidianus ambivalens
Mo 0.0025 mmol Mo per g protein Acidianus ambivalens
Ni 0.0025 mmol Ni per g protein Acidianus ambivalens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 x * 41000 + x * 66000, SDS-PAGE Acidianus ambivalens
66000
-
 x * 41000 + x * 66000, SDS-PAGE Acidianus ambivalens

Organism

Organism UniProt Comment Textmining
Acidianus ambivalens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
density-gradient centrifugation, column chromatography Acidianus ambivalens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
114
-
 acceptor methyl viologen Acidianus ambivalens
624
-
 acceptor 2,3-dimethylnaphthoquinol Acidianus ambivalens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + oxidized 2,3-dimethylnaphthoquinone
-
 Acidianus ambivalens 2,3-dimethylnaphthoquinol
-
 ?
H2 + oxidized benzyl viologen
-
 Acidianus ambivalens reduced benzyl viologen + H+
-
 ?
H2 + oxidized methyl viologen
-
 Acidianus ambivalens reduced methyl viologen + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 41000 + x * 66000, SDS-PAGE Acidianus ambivalens