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Literature summary for 1.12.99.6 extracted from

  • Seefeldt, L.C.; Arp, D.J.
    Oxygen effects on the nickel- and iron-containing hydrogenase from Azotobacter vinelandii (1989), Biochemistry, 28, 1588-1596.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
O2 non competitive versus methylene blue, uncompetitive versus H2, reversibility is time-dependent, inhibition is protected by H2 Azotobacter vinelandii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
H2
-
Azotobacter vinelandii
0.017
-
oxidized methylene blue
-
Azotobacter vinelandii

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Azotobacter vinelandii 16020
-

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Azotobacter vinelandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + acceptor methylene blue acts as electron acceptor Azotobacter vinelandii H+ + reduced acceptor
-
?
H2 + oxidized methylene blue
-
Azotobacter vinelandii reduced methylene blue
-
r

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0025
-
O2 membrane-associated enzyme Azotobacter vinelandii
0.0055
-
O2 purified enzyme Azotobacter vinelandii