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Literature summary for 1.12.99.6 extracted from
- Characterization of hydrogenase from the hyperthermophilic archaebacterium, Pyrococcus furiosus (1989), J. Biol. Chem., 264, 5070-5079.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| O2 | reversible | Pyrococcus furiosus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
H2 | with methylene blue as electron acceptor | Pyrococcus furiosus | |
| 1.9 | - |
H2 | with methyl viologen as electron acceptor | Pyrococcus furiosus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Pyrococcus furiosus | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | 31 g iron per 185 g enzyme | Pyrococcus furiosus | |
| Ni | 31 g nickel per 185 g enzyme | Pyrococcus furiosus | |
| Sulfide | 24 g acid labile sulfide per 185 g enzyme | Pyrococcus furiosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
alpha2,beta2,gamma2, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
| 27000 | - |
alpha2,beta2,gamma2, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
| 46000 | - |
alpha2,beta2,gamma2, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
| 185000 | - |
gel filtration | Pyrococcus furiosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + acceptor | Pyrococcus furiosus | ferredoxin acts as physiological electron donor | H+ + reduced acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | - |
strain DSM 3638 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| apparent homogeneity | Pyrococcus furiosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 360 | - |
- |
Pyrococcus furiosus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -196°C no loss of activity after freezing and thawing under argon | Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + acceptor | methyl viologen acts as electron acceptor | Pyrococcus furiosus | H+ + reduced acceptor | - |
r | |
| H2 + acceptor | methylene blue acts as electron acceptor | Pyrococcus furiosus | H+ + reduced acceptor | - |
r | |
| H2 + acceptor | reduced ferredoxin can act as acceptor | Pyrococcus furiosus | H+ + reduced acceptor | - |
r | |
| H2 + acceptor | ferredoxin acts as physiological electron donor | Pyrococcus furiosus | H+ + reduced acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | alpha2,beta2,gamma2, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
above | Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
no loss of activity after 2 months under anaerobic conditions | Pyrococcus furiosus |
| 80 | - |
50% loss of activity after 23 h | Pyrococcus furiosus |
| 90 | - |
50% loss of activity after 3 h | Pyrococcus furiosus |
| 100 | - |
50% loss of activity after 2 h | Pyrococcus furiosus |
| 105 | - |
50% loss of activity after 5 min | Pyrococcus furiosus |
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