Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Pyrococcus furiosus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | contains 21 atoms iron per mol enzyme | Pyrococcus furiosus | |
Ni2+ | contains 0.9 atoms nickel per mol enzyme | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
30000 | - |
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
39000 | - |
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
52000 | - |
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
320000 | - |
gel filtration | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | E7FHC4 and E7FHN9 and E7FHW8 and E7FHF8 | subunits alpha, beta, gamma and delta | - |
Purification (Comment) | Organism |
---|---|
DEAE column chromatography, hydroxyapatite column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + benzyl viologen | highest activity | Pyrococcus furiosus | H2S + reduced benzyl viologen | - |
? | |
H2 + methyl viologen | - |
Pyrococcus furiosus | H2S + reduced methyl viologen | - |
? | |
H2 + polysulfide(n) | - |
Pyrococcus furiosus | H2S + polysulfide(n-1) | - |
r | |
additional information | ferredoxin from Pyrococcus furiosus is not an efficient electron carrier | Pyrococcus furiosus | ? | - |
? | |
NADPH + H+ + polysulfide(n) | - |
Pyrococcus furiosus | NADP+ + polysulfide(n-1) | - |
r |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
H-II | - |
Pyrococcus furiosus |
hydrogenase II | - |
Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains 0.83 FAD per mol enzyme | Pyrococcus furiosus | |
NADH | NADPH and NADH are equally efficient as electron donors for H2 production | Pyrococcus furiosus | |
NADPH | NADPH and NADH are equally efficient as electron donors for H2 production | Pyrococcus furiosus | |
[2Fe-2S]-center | contains 1 [2Fe-2S] center | Pyrococcus furiosus | |
[4Fe-4S]-center | contains 2 [4Fe-4S]-centers | Pyrococcus furiosus |