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Literature summary for 1.12.98.4 extracted from
- Hydrogen-sulfur oxidoreductase complex from Pyrodictium abyssi (2001), Methods Enzymol., 331, 442-451.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no quinione, but enzyme complex contains cytochrome c and has two additional cytochromes, probably of b-type | Pyrodictium abyssi |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu | 1.2 mol per mol protein | Pyrodictium abyssi |  |
| Fe | 50-55 mol iron per mol protein | Pyrodictium abyssi | |
| Mo | not detected | Pyrodictium abyssi | |
| Ni | 1.6 mol per mol protein | Pyrodictium abyssi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
enzym complex, gel-filtration | Pyrodictium abyssi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrodictium abyssi | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| the sulfur-reducing activity is irreversibly damaged by oxygen, anaerobic conditions: no loss of activity after one week, aerobic conditions: 80% loss of activity in 24 h | Pyrodictium abyssi |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| enzyme complex | Pyrodictium abyssi |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| H2 + (sulfide)n = hydrogen sulfide + (sulfide)n-1 | a sulfur-reducing complex including hydrogenase, a sulfur reductase and electron-transferring components described | Pyrodictium abyssi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.56 | - |
at 100°C | Pyrodictium abyssi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sulfur + electron donor | electron donor: molecular hydrogen | Pyrodictium abyssi | H2S + oxidized electron donor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | nine major polypeptides with molecular masses of 82000, 72000, 65000, 50000, 47000, 42000, 40000, 30000 and 24000 Da | Pyrodictium abyssi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
below 85°C about 10% loss of activity | Pyrodictium abyssi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Pyrodictium abyssi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.5 | - |
Pyrodictium abyssi |
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Release 2023.1 (January 2023)
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