Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.12.98.4 extracted from

  • Fauque, G.D.; Klimmek, O.; Kroger, A.
    Sulfur reductases from spirilloid mesophilic sulfur-reducing eubacteria (1994), Methods Enzymol., 243, 367-383.
No PubMed abstract available
Search for more literature for 1.12.98.4

Activating Compound

Activating Compound Comment Organism Structure
GSH
-
 Sulfurospirillum deleyianum
additional information no cytochrome c3, c7 or b involved Wolinella succinogenes
additional information no cytochrome c3, c7 or b involved Sulfurospirillum deleyianum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Sulfurospirillum deleyianum 16020
-
membrane oriented toward the periplasmic side Wolinella succinogenes 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe iron-sulfur (non-heme) protein Wolinella succinogenes
Fe iron-sulfur (non-heme) protein Sulfurospirillum deleyianum
Fe 0.12 mM nonheme iron per g protein, Psr B (Fe-S)-centers Wolinella succinogenes
Mo enzym contains molybdenum 0.006 mmol/g protein bound to a pterin dinucleotide Wolinella succinogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sulfur Wolinella succinogenes reduction is coupled to the phosphorylation of ADP with phosphate in the catabolism of some anaerobic microorganisms H2S
-
 ?
sulfur Sulfurospirillum deleyianum reduction is coupled to the phosphorylation of ADP with phosphate in the catabolism of some anaerobic microorganisms H2S
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Triton X-100
-
 Wolinella succinogenes
Triton X-100
-
 Sulfurospirillum deleyianum

Organism

Organism UniProt Comment Textmining
Sulfurospirillum deleyianum
-
-
-
Wolinella succinogenes
-
 DSM 1740
-
Wolinella succinogenes
-
 enzyme formed during growth at the expense of sulfur reduction by formate
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sulfurospirillum deleyianum
isolation and incorporation into liposomes Wolinella succinogenes

Reaction

Reaction Comment Organism Reaction ID
H2 + (sulfide)n = hydrogen sulfide + (sulfide)n-1 sulfur can be reduced with hydrogen as donor in the presence of hydrogenase Wolinella succinogenes
a
H2 + (sulfide)n = hydrogen sulfide + (sulfide)n-1 sulfur can be reduced with hydrogen as donor in the presence of hydrogenase Sulfurospirillum deleyianum
a
H2 + (sulfide)n = hydrogen sulfide + (sulfide)n-1 polysulfide appears to be the soluble intermediate in the sulfur reduction Wolinella succinogenes
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.27
-
 crude extract Sulfurospirillum deleyianum
732
-
-
 Wolinella succinogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information sulfur can be reduced by photochemical reduction in the presence of phenosafranin Sulfurospirillum deleyianum ?
-
 ?
organic trisulfides + electron donor
-
 Sulfurospirillum deleyianum H2S + oxidized electron donor
-
 ?
polysulfide + electron donor
-
 Sulfurospirillum deleyianum H2S + oxidized electron donor
-
 ?
polysulfide + electron donor polysulfide appears to be the soluble intermediate in the sulfur reduction, electron-transport-coupled phosphorylation Wolinella succinogenes H2S + oxidized electron donor
-
 ?
sulfur reduction is coupled to the phosphorylation of ADP with phosphate in the catabolism of some anaerobic microorganisms Wolinella succinogenes H2S
-
 ?
sulfur reduction is coupled to the phosphorylation of ADP with phosphate in the catabolism of some anaerobic microorganisms Sulfurospirillum deleyianum H2S
-
 ?
sulfur + electron donor
-
 Sulfurospirillum deleyianum H2S + oxidized electron donor
-
 ?
sulfur + electron donor electron donor: molecular hydrogen Wolinella succinogenes H2S + oxidized electron donor
-
 ?

Subunits

Subunits Comment Organism
More SDS-PAGE, Psr A: 85000 Da and Psr B: 23000 Da, amount to approximately 70% of the total protein and are present at nearly equimolar quantities, Psr C not identified Wolinella succinogenes

Synonyms

Synonyms Comment Organism
polysulfide reductase Psr A, Psr B, Psr C Wolinella succinogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7 8.9
-
 Sulfurospirillum deleyianum