Cloned (Comment) | Organism |
---|---|
- |
Methanothermococcus thermolithotrophicus |
General Stability | Organism |
---|---|
50% loss of activity after freezing and thawing | Methanothermococcus thermolithotrophicus |
the rate of inactivation increases upon dilution and in the presence of O2 and decreases upon the addition of 5 mg/ml albumin | Methanothermococcus thermolithotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
N5,N10-methylenetetrahydromethanopterin | - |
Methanothermococcus thermolithotrophicus | |
0.15 | - |
H2 | - |
Methanothermococcus thermolithotrophicus | |
0.85 | - |
N5,N10-methenyltetrahydromethanopterin | - |
Methanothermococcus thermolithotrophicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not contain nickel or iron/sulfur clusters | Methanothermobacter thermautotrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37992 | - |
4 * 37992, calculation from nucleotide sequence | Methanothermococcus thermolithotrophicus |
38038 | - |
4 * 38038, MALDI-TOF-MS analysis of peptides obtained by BrCN cleavage | Methanothermococcus thermolithotrophicus |
43000 | - |
4 * 43000, SDS-PAGE | Methanothermococcus thermolithotrophicus |
150000 | - |
gel filtration | Methanothermococcus thermolithotrophicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Methanothermococcus thermolithotrophicus | - |
- |
- |
Oxidation Stability | Organism |
---|---|
purified enzyme is very labile even under strictly anoxic conditions | Methanothermococcus thermolithotrophicus |
Purification (Comment) | Organism |
---|---|
- |
Methanothermococcus thermolithotrophicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
440 | - |
- |
Methanothermococcus thermolithotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N5,N10-methylenetetrahydromethanopterin + H+ | r | Methanothermobacter thermautotrophicus | N5,N10-methenyltetrahydromethanopterin + H2 | - |
? | |
N5,N10-methylenetetrahydromethanopterin + H+ | r | Methanothermococcus thermolithotrophicus | N5,N10-methenyltetrahydromethanopterin + H2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 43000, SDS-PAGE | Methanothermococcus thermolithotrophicus |
tetramer | 4 * 37992, calculation from nucleotide sequence | Methanothermococcus thermolithotrophicus |
tetramer | 4 * 38038, MALDI-TOF-MS analysis of peptides obtained by BrCN cleavage | Methanothermococcus thermolithotrophicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Methanothermococcus thermolithotrophicus |