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Literature summary for 1.12.98.1 extracted from

  • Fiebig, K.; Friedrich, B.
    Purification of the F420-reducing hydrogenase from Methanosarcina barkeri (strain Fusaro) (1989), Eur. J. Biochem., 184, 79-88.
    View publication on PubMed
Search for more literature for 1.12.98.1

Activating Compound

Activating Compound Comment Organism Structure
additional information conditions for anaerobic reductive activation in the presence of hydrogen, 2-mercaptoethanol and KCl or methyl viologen yield maximal hydrogenase activity Methanosarcina barkeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0033
-
 oxidized methyl viologen 37°C, pH 8.3 Methanosarcina barkeri
0.025
-
 oxidized coenzyme F420 37°C, pH 7.5 Methanosarcina barkeri

Metals/Ions

Metals/Ions Comment Organism Structure
KCl increases activity, maximal activity at 250 mM Methanosarcina barkeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
8000
-
 x * 8000 + x * 33000 + x * 30000, SDS-PAGE Methanosarcina barkeri
30000
-
 x * 8000 + x * 33000 + x * 30000, SDS-PAGE Methanosarcina barkeri
33000
-
 x * 8000 + x * 33000 + x * 30000, SDS-PAGE Methanosarcina barkeri
198000
-
 non-denaturing PAGE, small enzyme form Methanosarcina barkeri
845000
-
 non-denaturing PAGE, large enzyme form Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2 + oxidized coenzyme F420 Methanosarcina barkeri
-
 reduced coenzyme F420
-
 ?
H2 + oxidized coenzyme F420 Methanosarcina barkeri DSM 804
-
 reduced coenzyme F420
-
 ?
H2 + oxidized methyl viologen Methanosarcina barkeri
-
 reduced methyl viologen
-
 ?
H2 + oxidized methyl viologen Methanosarcina barkeri DSM 804
-
 reduced methyl viologen
-
 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri P80490 and P80489 and P80491 P80490: subunit beta, P80489: subunit alpha, P80491: subunit gamma
-
Methanosarcina barkeri DSM 804 P80490 and P80489 and P80491 P80490: subunit beta, P80489: subunit alpha, P80491: subunit gamma
-

Oxidation Stability

Oxidation Stability Organism
enzyme activity is unstable under reducing conditions Methanosarcina barkeri

Purification (Commentary)

Purification (Comment) Organism
-
 Methanosarcina barkeri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.5
-
 37°C, pH 7.5, substrate: oxidized coenzyme F420 Methanosarcina barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + oxidized coenzyme F420
-
 Methanosarcina barkeri reduced coenzyme F420
-
 ?
H2 + oxidized coenzyme F420
-
 Methanosarcina barkeri DSM 804 reduced coenzyme F420
-
 ?
H2 + oxidized methyl viologen
-
 Methanosarcina barkeri reduced methyl viologen
-
 ?
H2 + oxidized methyl viologen
-
 Methanosarcina barkeri DSM 804 reduced methyl viologen
-
 ?

Subunits

Subunits Comment Organism
? x * 8000 + x * 33000 + x * 30000, SDS-PAGE Methanosarcina barkeri

Synonyms

Synonyms Comment Organism
F420-reducing hydrogenase
-
 Methanosarcina barkeri
Mbar_A0449 subunit beta Methanosarcina barkeri
Mbar_A0450 subunit gamma Methanosarcina barkeri
Mbar_A0452 subunit alpha Methanosarcina barkeri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
353
-
 oxidized coenzyme F420 37°C, pH 7.5 Methanosarcina barkeri
9226
-
 oxidized methyl viologen 37°C, pH 8.3 Methanosarcina barkeri