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Literature summary for 1.12.98.1 extracted from
- Biochemical characterization of the 8-hydroxy-5-deazaflavin-reactive hydrogenase from Methanosarcina barkeri Fusaro (1995), Eur. J. Biochem., 233, 727-735.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
H2 | 37°C, pH 7.2, cosubstrate: oxidized coenzyme F420 | Methanosarcina barkeri |  |
| 0.004 | - |
H2 | 37°C, pH 7.2, cosubstrate: oxidized methyl viologen | Methanosarcina barkeri | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | 1 mol of the 198000 Da enzyme form contains 28-32 mol of non-heme iron | Methanosarcina barkeri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
2 * 48000 (alpha) + 2 * 33000 (beta) + 2 * 30000 (gamma) | Methanosarcina barkeri |
| 33000 | - |
2 * 48000 (alpha) + 2 * 33000 (beta) + 2 * 30000 (gamma) | Methanosarcina barkeri |
| 48000 | - |
2 * 48000 (alpha) + 2 * 33000 (beta) + 2 * 30000 (gamma) | Methanosarcina barkeri |
| 198000 | - |
- |
Methanosarcina barkeri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + oxidized coenzyme F420 | Methanosarcina barkeri | - |
reduced coenzyme F420 | - |
? | |
| H2 + oxidized coenzyme F420 | Methanosarcina barkeri DSM 804 | - |
reduced coenzyme F420 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina barkeri | P80490 and P80489 and P80491 | P80490: subunit beta, P80489: subunit alpha, P80491: subunit gamma | - |
| Methanosarcina barkeri DSM 804 | P80490 and P80489 and P80491 | P80490: subunit beta, P80489: subunit alpha, P80491: subunit gamma | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanosarcina barkeri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 82.8 | - |
37°C, pH 7.2, cosubstrate: oxidized coenzyme F420 | Methanosarcina barkeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + oxidized benzyl viologen | - |
Methanosarcina barkeri | reduced benzyl viologen | - |
? | |
| H2 + oxidized benzyl viologen | - |
Methanosarcina barkeri DSM 804 | reduced benzyl viologen | - |
? | |
| H2 + oxidized coenzyme F0 | cofactor F0 i.e. 7,8-didemethy1-8-hydroxy-S-deazaflavin | Methanosarcina barkeri | reduced coenzyme F20 | - |
? | |
| H2 + oxidized coenzyme F0 | cofactor F0 i.e. 7,8-didemethy1-8-hydroxy-S-deazaflavin | Methanosarcina barkeri DSM 804 | reduced coenzyme F20 | - |
? | |
| H2 + oxidized coenzyme F420 | - |
Methanosarcina barkeri | reduced coenzyme F420 | - |
? | |
| H2 + oxidized coenzyme F420 | - |
Methanosarcina barkeri DSM 804 | reduced coenzyme F420 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 2 * 48000 (alpha) + 2 * 33000 (beta) + 2 * 30000 (gamma) | Methanosarcina barkeri |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 8-hydroxy-5-deazaflavin-reactive hydrogenase | - |
Methanosarcina barkeri |
| F420-reducing hydrogenase | - |
Methanosarcina barkeri |
| Mbar_A0449 | subunit beta | Methanosarcina barkeri |
| Mbar_A0450 | subunit gamma | Methanosarcina barkeri |
| Mbar_A0452 | subunit alpha | Methanosarcina barkeri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Methanosarcina barkeri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.25 | deazaflavin reducing activity | Methanosarcina barkeri |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10 | the methylviologen-reducing activity increased with pH from pH 5.5-10 | Methanosarcina barkeri |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | 1 mol of the 198000 Da enzyme form contains 2 mol of FAD | Methanosarcina barkeri | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Methanosarcina barkeri | isoelectric focusing | - |
5.3 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 25000 | - |
H2 | 37°C, pH 7.2, cosubstrate: oxidized coenzyme F420 | Methanosarcina barkeri | |
| 69000 | - |
H2 | 37°C, pH 7.2, cosubstrate: oxidized methyl viologen | Methanosarcina barkeri | |
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