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Literature summary for 1.12.98.1 extracted from

  • Shah, N.N.; Clark, D.S.
    Partial purification and characterization of two hydrogenases from the extreme thermophile Methanococcus jannaschii (1990), Appl. Environ. Microbiol., 56, 858-863.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information 2 times higher activity in biological buffers like Tris or EPPS compared with phosphate buffer Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
smallest species with enzymatic activity, native SDS-PAGE Methanocaldococcus jannaschii
990000
-
native SDS-PAGE Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2 + coenzyme F420 Methanocaldococcus jannaschii enzyme of methanogenesis pathway reduced coenzyme F420
-
?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.2
-
purified enzyme with F420 Methanocaldococcus jannaschii
16
-
purified enzyme with methyl viologen Methanocaldococcus jannaschii

Storage Stability

Storage Stability Organism
-70°C, loss of high temperature activity Methanocaldococcus jannaschii
4°C, 5% loss of activity within 24 h Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + coenzyme F420
-
Methanocaldococcus jannaschii reduced coenzyme F420
-
?
H2 + coenzyme F420 enzyme of methanogenesis pathway Methanocaldococcus jannaschii reduced coenzyme F420
-
?
H2 + methyl viologen
-
Methanocaldococcus jannaschii ?
-
?

Subunits

Subunits Comment Organism
multimer alpha,beta,gamma 48000 + 32000 + 25000, SDS-PAGE, exact subunit composition not known Methanocaldococcus jannaschii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80 90
-
Methanocaldococcus jannaschii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 90 F420 reduction Methanocaldococcus jannaschii
40 103 at 103°C low activity with methyl viologen Methanocaldococcus jannaschii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
stable for more than 3 h, methyl viologen-linked activity more stable against heating than F420-linked activity Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
F420 reduction Methanocaldococcus jannaschii
10
-
optimum pH for methyl viologen reduction above pH 10 Methanocaldococcus jannaschii

pH Stability

pH Stability pH Stability Maximum Comment Organism
10
-
unstable above pH 10 Methanocaldococcus jannaschii