Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | 2 times higher activity in biological buffers like Tris or EPPS compared with phosphate buffer | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
115000 | - |
smallest species with enzymatic activity, native SDS-PAGE | Methanocaldococcus jannaschii |
990000 | - |
native SDS-PAGE | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + coenzyme F420 | Methanocaldococcus jannaschii | enzyme of methanogenesis pathway | reduced coenzyme F420 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Methanocaldococcus jannaschii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.2 | - |
purified enzyme with F420 | Methanocaldococcus jannaschii |
16 | - |
purified enzyme with methyl viologen | Methanocaldococcus jannaschii |
Storage Stability | Organism |
---|---|
-70°C, loss of high temperature activity | Methanocaldococcus jannaschii |
4°C, 5% loss of activity within 24 h | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + coenzyme F420 | - |
Methanocaldococcus jannaschii | reduced coenzyme F420 | - |
? | |
H2 + coenzyme F420 | enzyme of methanogenesis pathway | Methanocaldococcus jannaschii | reduced coenzyme F420 | - |
? | |
H2 + methyl viologen | - |
Methanocaldococcus jannaschii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | alpha,beta,gamma 48000 + 32000 + 25000, SDS-PAGE, exact subunit composition not known | Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 90 | - |
Methanocaldococcus jannaschii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 90 | F420 reduction | Methanocaldococcus jannaschii |
40 | 103 | at 103°C low activity with methyl viologen | Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
stable for more than 3 h, methyl viologen-linked activity more stable against heating than F420-linked activity | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
F420 reduction | Methanocaldococcus jannaschii |
10 | - |
optimum pH for methyl viologen reduction above pH 10 | Methanocaldococcus jannaschii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
10 | - |
unstable above pH 10 | Methanocaldococcus jannaschii |