Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
gel filtration | Methanobacterium formicicum |
380000 | - |
gel filtration | Methanobacterium formicicum |
600000 | - |
gel filtration | Methanobacterium formicicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + coenzyme F420 | Methanobacterium bryantii | enzyme of methanogenesis pathway | reduced coenzyme F420 | - |
? | |
H2 + coenzyme F420 | Methanobacterium formicicum | enzyme of methanogenesis pathway | reduced coenzyme F420 | - |
? | |
H2 + coenzyme F420 | Methanobacterium formicicum MF | enzyme of methanogenesis pathway | reduced coenzyme F420 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanobacterium bryantii | - |
- |
- |
Methanobacterium formicicum | - |
- |
- |
Methanobacterium formicicum MF | - |
- |
- |
Oxidation Stability | Organism |
---|---|
more stable under aerobic conditions, reversible loss of activity with oxygen can be restored with dithionite | Methanobacterium formicicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0014 | - |
methyl viologen-linked activity | Methanobacterium bryantii |
0.0026 | - |
methyl viologen-linked activity | Methanobacterium formicicum |
0.032 | - |
F420-linked activity | Methanobacterium formicicum |
0.155 | - |
F420-linked activity | Methanobacterium bryantii |
Storage Stability | Organism |
---|---|
4°C, 4 weeks, 25% loss of activity towards F420, 97% loss of activity towards methyl viologen | Methanobacterium formicicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + coenzyme F420 | - |
Methanobacterium bryantii | reduced coenzyme F420 | - |
? | |
H2 + coenzyme F420 | - |
Methanobacterium formicicum | reduced coenzyme F420 | - |
? | |
H2 + coenzyme F420 | enzyme of methanogenesis pathway | Methanobacterium bryantii | reduced coenzyme F420 | - |
? | |
H2 + coenzyme F420 | enzyme of methanogenesis pathway | Methanobacterium formicicum | reduced coenzyme F420 | - |
? | |
H2 + coenzyme F420 | - |
Methanobacterium formicicum MF | reduced coenzyme F420 | - |
? | |
H2 + coenzyme F420 | enzyme of methanogenesis pathway | Methanobacterium formicicum MF | reduced coenzyme F420 | - |
? | |
H2 + methyl viologen | - |
Methanobacterium bryantii | ? | - |
? | |
H2 + methyl viologen | - |
Methanobacterium formicicum | ? | - |
? | |
H2 + methyl viologen | - |
Methanobacterium formicicum MF | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | alpha,beta,gamma 42600 + 23500 + 35000, SDS-PAGE, exact subunit composition not known | Methanobacterium formicicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
5 | reactivated enzyme stable | Methanobacterium formicicum |
25 | 30 | reactivated enzyme unstable | Methanobacterium formicicum |